LOCUS       sequence-141741-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Gap43. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Hirota J, Mombaerts P
  TITLE     The LIM-homeodomain protein Lhx2 is required for complete 
            development of mouse olfactory sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jun 8;101(23):8751-5. Epub 2004 Jun 
            1.
  PUBMED    15173589
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2004-06-8"; volume: "101(23)"; pages: 
            "8751-5. Epub 2004 Jun 1"
COMMENT     Sequence Label: pISH-Gap43
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      770..779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      774..783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(813..832)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(814..832)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(839..856)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(839..855)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(848..870)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tatggtcgac ctgcaggcgg ccgcgaattc actagtgatt gcaacgtgga aagccatttc
       61 ttaaagttca ggcatgttct tggtcagcct cggggtcttc tttaccctca tcctgtcggg
      121 cactttcctt aggtttggct tcgtctacag cgtctttctc ctcctcagct tggctgcttt
      181 ctgcagtctc cgttggaggc tgcgctgcct tggtggcagc atcctctgca gcaggggtgg
      241 tggcagcagc atcagtgaca gcagcaggca catcggcttg tttaggctcc tccttggctg
      301 ggccatcttc agccttggag gacggggagt tatcagtggt agctttagca gcactttctg
      361 tctccgctga gccggccttt tcctcggagg gggccgcatc cccttcaccc ttcttctcct
      421 cagaaggtgc atctcctgcc ttgctgggct cctcagcctt ggggctggtg gctggggctg
      481 catcggtagt agcagagcca tctccctcct tcttctccac accatcagca acgggagcat
      541 catccttctc cttggcctcg gcctcagcag ctggtgcatc acccttcttc tcgcctttga
      601 gctttttcct tgttatgtgt ccacggaagc tagcctgaat tttggtcgca gccttatgag
      661 ccttatcttc cggcttgaca ccatcttgtt caatcatttg gtcctcatca ttcttttcaa
      721 cctgtttggt tcttctcata cagcacagca tggtaatcga attcccgcgg ccgccatggc
      781 ggccgggagc atgcgacgtc gggcccaatt cgccctatag tgagtcgtat tacaattcac
      841 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      901 ttgca
//
