LOCUS       sequence-141744-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Gap43. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Hirota J, Mombaerts P
  TITLE     The LIM-homeodomain protein Lhx2 is required for complete 
            development of mouse olfactory sensory neurons.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jun 8;101(23):8751-5. Epub 2004 Jun 
            1.
  PUBMED    15173589
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2004-06-8"; volume: "101(23)"; pages: 
            "8751-5. Epub 2004 Jun 1"
COMMENT     Sequence Label: pISH-Gap43
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(813..831)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(814..831)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(849..865)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(849..865)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(862..884)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    873..889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(897..903,904..921,922..927))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    942..963
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggccgcggga attcgattac catgctgtgc tgtatgagaa gaaccaaaca ggttgaaaag
       61 aatgatgagg accaaatgat tgaacaagat ggtgtcaagc cggaagataa ggctcataag
      121 gctgcgacca aaattcaggc tagcttccgt ggacacataa caaggaaaaa gctcaaaggc
      181 gagaagaagg gtgatgcacc agctgctgag gccgaggcca aggagaagga tgatgctccc
      241 gttgctgatg gtgtggagaa gaaggaggga gatggctctg ctactaccga tgcagcccca
      301 gccaccagcc ccaaggctga ggagcccagc aaggcaggag atgcaccttc tgaggagaag
      361 aagggtgaag gggatgcggc cccctccgag gaaaaggccg gctcagcgga gacagaaagt
      421 gctgctaaag ctaccactga taactccccg tcctccaagg ctgaagatgg cccagccaag
      481 gaggagccta aacaagccga tgtgcctgct gctgtcactg atgctgctgc caccacccct
      541 gctgcagagg atgctgccac caaggcagcg cagcctccaa cggagactgc agaaagcagc
      601 caagctgagg aggagaaaga cgctgtagac gaagccaaac ctaaggaaag tgcccgacag
      661 gatgagggta aagaagaccc cgaggctgac caagaacatg cctgaacttt aagaaatggc
      721 tttccacgtt gcaatcacta gtgaattcgc ggccgcctgc aggtcgacca tatgggagag
      781 ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa tagcttggcg
      841 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      901 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      961 ttaattgcgt tgcgctcact gcccgctttc cagtcggga
//
