LOCUS       sequence-141745-         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express SMCR8 HA tagged in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  TITLE     pcDNA3 HA C9ORF72 op
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMV6 SMCR8 HA
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     173..189
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     173..189
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(209..232)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(248..274)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cttgctccat accacccccc tccaccccat aatattatag aaggacacct agtcagacaa
       61 aatgatgcaa cttaatttta ttaggacaag gctggtgggc actggagtgg caacttccag
      121 ggccaggaga ggcactgggg aggggtcaca gggatgccac ccgggatctg ttcaggaaac
      181 agctatgacc gcggccggcc gtttaaacct tatcgtcgtc atccttgtaa tccaggatat
      241 cttacttagc gtaatctgga acatcgtatg ggtactcgag cggccgcgta cgcgtgattt
      301 tatacaaaaa gctggggaca tagtcaaacc tgagcatggg gtggctggtc cctggagatt
      361 cctgcatgta gtgcagcttc agcagctcag ccaggtactg gaccacccta acatcctcat
      421 tcaccagacc cagggtcagc tttaggaagc tcatctggcg gctggctacc agctcctctg
      481 tctccttgtc gtgggtaggc aggtgcaggt ggtggcagaa ggtgtagagg aaggccttgg
      541 agcagagctg ggtgagcatg ctctggacat gcaggtagta ggtgctgccc cgcttgatct
      601 gtgtgcggtg gtctgccagg cggggcacca gggtgcctct gtaaaggggg cagcgcaggg
      661 ttttgttgtc aaggtccagg atgctcgtgt a
//
