LOCUS       sequence-141747-         360 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin variant with mutations L69P and V70L reverted to wild type
            (i53-DM mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Canny MD, Moatti N, Wan LCK, Fradet-Turcotte A, Krasner D, 
            Mateos-Gomez PA, Zimmermann M, Orthwein A, Juang YC, Zhang W, 
            Noordermeer SM, Seclen E, Wilson MD, Vorobyov A, Munro M, Ernst A, 
            Ng TF, Cho T, Cannon PM, Sidhu SS, Sicheri F, Durocher D
  TITLE     Inhibition of 53BP1 favors homology-dependent DNA repair and 
            increases CRISPR-Cas9 genome-editing efficiency.
  JOURNAL   Nat Biotechnol. 2018 Jan;36(1):95-102. doi: 10.1038/nbt.4021. Epub 
            2017 Nov 27.
  PUBMED    29176614
REFERENCE   2  (bases 1 to 360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.4021"; 
            journalName: "Nat Biotechnol"; date: "2018-01"; volume: "36"; issue:
            "1"; pages: "95-102"
COMMENT     Sequence Label: pcDNA3-Flag::UbvG08 P69L, L70V, I44A, deltaGG
FEATURES             Location/Qualifiers
     source          1..360
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..63
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atacgactca ctatagggag acccaagctt ggtaccatgg actacaaaga cgatgacgat
       61 aaagccgcca gtttaaacgg cgcgccatta attaaggatc caatgttgat tttcgtgaaa
      121 acccttaccg ggaaaaccat caccctcgag gttgaaccct cggatacgat agaaaatgta
      181 aaggccaaga tccaggataa ggaaggaatt cctcctgatc agcagagact ggcctttgct
      241 ggcaaatcgc tggaagatgg acgtactttg tctgactaca atattctaaa ggactctaaa
      301 cttcatctag tgttgagact tcgttgagcg gccgctcgag catgcatcta gagggcccta
//
