LOCUS       sequence-141749-         225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ubiquitin variant that binds to and occludes the ligand binding site
            of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at 
            sites of DNA damage. Potent selective inhibitor of 53BP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Canny MD, Moatti N, Wan LCK, Fradet-Turcotte A, Krasner D, 
            Mateos-Gomez PA, Zimmermann M, Orthwein A, Juang YC, Zhang W, 
            Noordermeer SM, Seclen E, Wilson MD, Vorobyov A, Munro M, Ernst A, 
            Ng TF, Cho T, Cannon PM, Sidhu SS, Sicheri F, Durocher D
  TITLE     Inhibition of 53BP1 favors homology-dependent DNA repair and 
            increases CRISPR-Cas9 genome-editing efficiency.
  JOURNAL   Nat Biotechnol. 2018 Jan;36(1):95-102. doi: 10.1038/nbt.4021. Epub 
            2017 Nov 27.
  PUBMED    29176614
REFERENCE   2  (bases 1 to 225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.4021"; 
            journalName: "Nat Biotechnol"; date: "2018-01"; volume: "36"; issue:
            "1"; pages: "95-102"
COMMENT     Sequence Label: pcDNA3-Flag::UbvG08 I44A, deltaGG
FEATURES             Location/Qualifiers
     source          1..225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgttgattt tcgtgaaaac ccttaccggg aaaaccatca ccctcgaggt tgaaccctcg
       61 gatacgatag aaaatgtaaa ggccaagatc caggataagg aaggaattcc tcctgatcag
      121 cagagactgg cctttgctgg caaatcgctg gaagatggac gtactttgtc tgactacaat
      181 attctaaagg actctaaact tcatcctctg ttgagacttc gttga
//
