LOCUS       sequence-141751-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase
            with C-terminal His6 tag and Glu294>Ala mutant (AarCH6-E294A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Mullins EA, Kappock TJ
  TITLE     Crystal structures of Acetobacter aceti succinyl-coenzyme A 
            (CoA):acetate CoA-transferase reveal specificity determinants and 
            illustrate the mechanism used by class I CoA-transferases.
  JOURNAL   Biochemistry. 2012 Oct 23;51(42):8422-34. doi: 10.1021/bi300957f. 
            Epub 2012 Oct 11.
  PUBMED    23030530
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi300957f";
            journalName: "Biochemistry"; date: "2012-10-23- 23"; volume: "51"; 
            issue: "42"; pages: "8422-34"
COMMENT     Sequence Label: pJK513
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgacagag cgcattcgga acgtcgcgct
       61 gcgcagtaaa gtatgtccgg cagaaaccgc atccgagttg atcaagcacg gagacgtggt
      121 ggggaccagc ggttttaccg gggcaggtta ccccaaggaa gtacccaagg cgctggccca
      181 gcggatggaa gctgcgcatg atcggggcga aaaataccag atcagcctga ttacgggcgc
      241 atccacgggc cctcagctgg atggtgagct ggccaaggcc aacggggtgt atttccgctc
      301 gccattcaat acggatgcca ccatgcgcaa ccgtattaat gcgggtgaaa cggaatattt
      361 tgataaccat ctggggcagg tggctggccg tgccgtgcag ggcaattacg gcaagttcaa
      421 tattgcgctg gtggaagcca cagccattac ggaagatggc ggcattgtgc ccacatcttc
      481 tgtcggcaat tcccagacat tcctcaatct ggccgaaaag gtcattatcg aggtaaatga
      541 atggcagaat ccgatgctgg aaggcatcca tgatatctgg gatggcaacg taagtggcgt
      601 gcctacgcgt gatattgtgc cgattgtacg ggcagatcag cgcgtgggtg gcccggtttt
      661 acgggtgaac ccggacaaga ttgctgccat tgtacgcacg aatgaccggg acagaaatgc
      721 cccgtttgcc gcgccggatg aaacagcaaa agctattgcg ggctatttgc ttgatttctt
      781 tgggcatgaa gtcaaacaga accgtttgcc gccctcactt ctgccgctcc agtctggtgt
      841 gggcaatgtg gccaacgccg tgctggaagg gctgaaggaa ggcccatttg aaaatctggt
      901 gggctatagt gcggtgattc aggatggcat gttggccatg ctggattctg gccgtatgcg
//
