LOCUS       sequence-141760-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Enhanced mutant version of membrane targeted PH-miniSOG (Q103L) 
            entry clone for more efficient ablation of cells when expressed 
            under desired promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Xu S, Chisholm AD
  TITLE     Highly efficient optogenetic cell ablation in C. elegans using 
            membrane-targeted miniSOG.
  JOURNAL   Sci Rep. 2016 Feb 10;6:21271. doi: 10.1038/srep21271.
  PUBMED    26861262
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-10"; volume: "6:21271. doi"; pages: " 
            10.1038/srep21271"
COMMENT     Sequence Label: pCZGY2849
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(136..163)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(255..341)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     390..412
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     404..421
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     405..421
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    437..536
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
       61 cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga
      121 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg
      181 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa
      241 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
      301 cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac
      361 tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag
      421 tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca
      481 acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag caggctccga
      541 attcgccctt cggatccaga tctatgcacg ggctccagga tgacccggac cttcaggccc
      601 ttctgaaggg cagccagctt ctgaaggtga agtccagctc gtggcgtagg gaacgcttct
      661 acaagctaca ggaggactgc aagaccatct ggcaggaatc tcgaaaggtc atgaggtccc
      721 cggagtcgca gctgttctcc atcgaggaca ttcaggaggt acggatggga caccgcacag
      781 aaggcctgga gaagtttgcc cgagacatcc ccgaggatcg atgcttctcc attgtcttca
      841 aggaccagcg caacacccta gacctcattg ccccatcacc agctgacgct cagcactggg
//
