LOCUS       sequence-141761-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Enhanced mutant version of membrane targeted PH-miniSOG (Q103L) 
            entry clone for more efficient ablation of cells when expressed 
            under desired promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Xu S, Chisholm AD
  TITLE     Highly efficient optogenetic cell ablation in C. elegans using 
            membrane-targeted miniSOG.
  JOURNAL   Sci Rep. 2016 Feb 10;6:21271. doi: 10.1038/srep21271.
  PUBMED    26861262
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-10"; volume: "6:21271. doi"; pages: " 
            10.1038/srep21271"
COMMENT     Sequence Label: pCZGY2849
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     382..398
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     382..398
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     403..422
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        403..421
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    439..538
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
       61 ggcatagact gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
      121 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
      181 cattacagct tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc
      241 cacggtgtgc gtcacccggc aaccttgggt agcagcgaag tcgaggcatt tctgtcctgg
      301 ctggtctaga ggaggcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
      361 tgagacacgg gccagagctg ccaggaaaca gctatgacca tgtaatacga ctcactatag
      421 gggatatcag ctggatggca aataatgatt ttattttgac tgatagtgac ctgttcgttg
      481 caacaaattg atgagcaatt attttttata atgccaactt tgtacaagaa agctgggtcg
      541 aattcgccct tgtcatccat ccagcagcac tccgatgaaa tactggagct cacccttctg
      601 gtccctcatg ggctggaggt gcaggaggtt ccagaatttc ttcccgcttt ttgtgtagtt
      661 tatcagctgc acggtaatct cgcgctgatc tctaatggca tcgcgaatct tctgcactgt
      721 ggcctggtct gtctctggtc cctgcagaaa gcggccattg cggcccagga tctcctctct
      781 ggaatactcg gtcagctcca ggaagccatc ggatgcgaag atgatgggat tgtctggcag
      841 ccgtggatca gttatcacga aacttttctc actagtcttc tgccgctggt ccatggagcc
      901 ggagtggtgg atgatcttgc gcaggccctg cacccagtgc tga
//
