LOCUS       sequence-141763-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Membrane targeted PH-miniSOG entry clone for efficient ablation of 
            cells when expressed under desired promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Xu S, Chisholm AD
  TITLE     Highly efficient optogenetic cell ablation in C. elegans using 
            membrane-targeted miniSOG.
  JOURNAL   Sci Rep. 2016 Feb 10;6:21271. doi: 10.1038/srep21271.
  PUBMED    26861262
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-10"; volume: "6:21271. doi"; pages: " 
            10.1038/srep21271"
COMMENT     Sequence Label: pCZGY2139
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(134..161)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(253..339)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     388..410
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     402..419
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     403..419
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    435..534
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
       61 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
      121 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
      181 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
      241 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
      301 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
      361 tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc
      421 ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac
      481 aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca ggctccgaat
      541 tcgcccttcg gatccagatc tatgcacggg ctccaggatg acccggacct tcaggccctt
      601 ctgaagggca gccagcttct gaaggtgaag tccagctcgt ggcgtaggga acgcttctac
      661 aagctacagg aggactgcaa gaccatctgg caggaatctc gaaaggtcat gaggtccccg
      721 gagtcgcagc tgttctccat cgaggacatt caggaggtac ggatgggaca ccgcacagaa
      781 ggcctggaga agtttgcccg agacatcccc gaggatcgat gcttctccat tgtcttcaag
      841 gaccagcgca acaccctaga cctcattgcc ccatcaccag ctgacgc
//
