LOCUS       sequence-141766-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Membrane targeted PH-miniSOG entry clone for efficient ablation of 
            cells when expressed under desired promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Xu S, Chisholm AD
  TITLE     Highly efficient optogenetic cell ablation in C. elegans using 
            membrane-targeted miniSOG.
  JOURNAL   Sci Rep. 2016 Feb 10;6:21271. doi: 10.1038/srep21271.
  PUBMED    26861262
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-10"; volume: "6:21271. doi"; pages: " 
            10.1038/srep21271"
COMMENT     Sequence Label: pCZGY2139
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     382..398
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     382..398
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     403..422
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        403..421
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    439..538
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(562..879)
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="EKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQGP
                     ETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQLD
                     G"
ORIGIN
        1 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
       61 ggcatagact gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
      121 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
      181 cattacagct tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc
      241 cacggtgtgc gtcacccggc aaccttgggt agcagcgaag tcgaggcatt tctgtcctgg
      301 ctggtctaga ggaggcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
      361 tgagacacgg gccagagctg ccaggaaaca gctatgacca tgtaatacga ctcactatag
      421 gggatatcag ctggatggca aataatgatt ttattttgac tgatagtgac ctgttcgttg
      481 caacaaattg atgagcaatt attttttata atgccaactt tgtacaagaa agctgggtcg
      541 aattcgccct tgttactcga gtcatccatc cagctgcact ccgatgaaat actggagctc
      601 acccttctgg tccctcatgg gctggaggtg caggaggttc cagaatttct tcccgctttt
      661 tgtgtagttt atcagctgca cggtaatctc gcgctgatct ctaatggcat cgcgaatctt
      721 ctgcactgtg gcctggtctg tctctggtcc ctgcagaaag cggccattgc ggcccaggat
      781 ctcctctctg gaatactcgg tcagctccag gaagccatcg gatgcgaaga tgatgggatt
      841 gtctggcagc cgtggatcag ttatcacgaa acttttctca ctagtcttct gccgctggtc
      901 catggagccg gagtggtgga tgatcttgcg caggccctgc acccagtgct gagcgtcagc
      961 tggtgatggg gcaatgaggt ctagggtgtt gcgctggtcc ttgaagacaa tggagaa
//
