LOCUS       sequence-141770-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express c-Myc-tagged human ASC in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Bryan NB, Dorfleutner A, Rojanasakul Y, Stehlik C
  TITLE     Activation of inflammasomes requires intracellular redistribution of
            the apoptotic speck-like protein containing a caspase recruitment 
            domain.
  JOURNAL   J Immunol. 2009 Mar 1;182(5):3173-82. doi: 10.4049/jimmunol.0802367.
  PUBMED    19234215
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4049/jimmunol"; 
            journalName: "J Immunol"; date: "2009-03-1- 1"; volume: "182"; 
            issue: "5"; pages: "3173-82"
COMMENT     Sequence Label: pcDNA3-Myc-ASC
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             107..136
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(806..824)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(807..824)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(844..861)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttcgatcgtc gacatggaac agaaactcat
      121 ctctgaagag gatctgtcta gaggatccga attcgatcct ggagccatgg ggcgcgcgcg
      181 cgacgccatc ctggatgcgc tggagaacct gaccgccgag gagctcaaga agttcaagct
      241 gaagctgctg tcggtgccgc tgcgcgaggg ctacgggcgc atcccgcggg gcgcgctgct
      301 gtccatggac gccttggacc tcaccgacaa gctggtcagc ttctacctgg agacctacgg
      361 cgccgagctc accgctaacg tgctgcgcga catgggcctg caggagatgg ccgggcagct
      421 gcaggcggcc acgcaccagg gctctggagc cgcgccagct gggatccagg cccctcctca
      481 gtcggcagcc aagccaggcc tgcactttat agaccagcac cgggctgcgc ttatcgcgag
      541 ggtcacaaac gttgagtggc tgctggatgc tctgtacggg aaggtcctga cggatgagca
      601 gtaccaggca gtgcgggccg agcccaccaa cccaagcaag atgcggaagc tcttcagttt
      661 cacaccagcc tggaactgga cctgcaagga cttgctcctc caggccctaa gggagtccca
      721 gtcctacctg gtggaggacc tggagcggag ctgaggctcc ttcccagcaa cactccggct
      781 cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
      841 cagcctcgac tgtgccttct agttgccagc catctgttgt t
//
