LOCUS       sequence-141775-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Adnp. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Adnp
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(608..626)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(609..626)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(644..660)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(644..660)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(657..679)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    668..684
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(692..698,699..716,717..722))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    737..758
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(875..892)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcggccgcgg gaattcgatt ccggttaaaa gttcacctca agctgcagtg ccctataaaa
       61 aagatgttgg gaagaccctt tgccctcttt gcttttcaat actaaaagga cccatatctg
      121 atgcacttgc acatcattta cgagaaagac accaagttat tcagacagtt catccggttg
      181 agaaaaagct aacttacaaa tgtatccatt gccttggtgt gtatactagc aacatgacag
      241 cctcaaccat cactctgcat ctagtccact gcaggggtgt tggaaaaacc cagaatggcc
      301 aggacaagac aaacgcacct tctcggctca atcagtctcc aggcctggcc cctgtgaagc
      361 gcacgtatga gcagatggag tttccactgc taaaaaagcg gaagctggag gaggatgctg
      421 attcccctag ctgctttgaa gagaagccag aagagcctgt tgttttagct ttagacccca
      481 agggtcatga agatgattct tatgaggcta ggaaaagctt tctcacaaat cactagtgaa
      541 ttcgcggccg cctgcaggtc gaccatatgg gagagctccc aacgcgttgg atgcatagct
      601 tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg
      661 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      721 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      781 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      841 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      901 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttat
//
