LOCUS       sequence-141784-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and 
            mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate 
            genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Chiang TW, le Sage C, Larrieu D, Demir M, Jackson SP
  TITLE     CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening 
            to enhance genome editing.
  JOURNAL   Sci Rep. 2016 Apr 15;6:24356. doi: 10.1038/srep24356.
  PUBMED    27079678
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-04-15"; volume: "6:24356. doi"; pages: " 
            10.1038/srep24356"
COMMENT     Sequence Label: AIO-mCherry
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(104..160)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(170..217)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga
       61 actccttgat gatggccatg ttatcctcct cgcccttgct cacaggaccg gggttttctt
      121 ccacgtctcc tgcttgcttt aacagagaga agttcgtggc tccggagccc tttttctttt
      181 ttgcctggcc ggcctttttc gtggccgccg gccttttgtc gcctcccagc tgagacaggt
      241 cgatccgtgt ctcgtacagg ccggtgatgc tctggtggat cagggtggcg tccagcacct
      301 ctttggtgct ggtgtacctc ttccggtcga tggtggtgtc aaagtacttg aaggcggcag
      361 gggctcccag attggtcagg gtaaacaggt ggatgatatt ctcggcctgc tctctgatgg
      421 gcttatcccg gtgcttgttg taggcggaca gcactttgtc cagattagcg tcggccagga
      481 tcactctctt ggagaactcg ctgatctgct cgatgatctc gtccaggtag tgcttgtgct
      541 gttccacaaa cagctgtttc tgctcattat cctcggggga gcccttcagc ttctcatagt
      601 ggctggccag gtacaggaag ttcacatatt tggagggcag ggccagttcg tttcccttct
      661 gcagttcgcc ggcagaggcc agcattctct tccggccgtt ttccagctcg aacagggagt
      721 acttaggcag cttgatgatc aggtcctttt tcacttcttt gtagcccttg gcttccagaa
      781 agtcgatggg attcttctcg aagctgcttc tttccatgat ggtgatcccc agcagctctt
      841 tcacactctt cagtttcttg gacttgccct tttccact
//
