LOCUS       sequence-141809-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Dlx5. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Dlx5
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(680..698)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(681..698)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(716..732)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(716..732)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(729..751)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    740..756
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(764..770,771..788,789..794))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    809..830
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cggccgcggg aattcgatta ttgatagtgt ccacagttgc gcaaaaaaaa aaaaaaaagg
       61 gttcttttcg taaaaaaggg ggcggggctc tctgaaatgc aataacttac atgcaaaaaa
      121 aagtttacac atgaatcttt tttgtttcca ttgttcaaac atccccgtat gaattccttt
      181 ctttctgatt tctaaaccag cacaacactg tagtcccaaa actgagcaag agaaagtagc
      241 ccatctaata aagcgtcccg gaggccagtg ccagcgggtg ctgcaaggag cccggcggtg
      301 gcaggtggga attgattgag ctggctgcgc ttgggtacca ggaagccgag ttctccaggt
      361 agctggacgc gggggactgg ttggaggtcg gagggtgggc atgagggtgg tggctgagag
      421 agcgggatga gccctggggc tcccacaccg ctggtgactg tggcgagtta cacgccatag
      481 ggtcgctgga gctgggactg tgctccgggg gcatctcccc gtttttcatg atcttcttga
      541 tcttggatct tttgttctga aaccagattt tcacctgtgt ttgcgtcagt cctagagaga
      601 atcactagtg aattcgcggc cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt
      661 ggatgcatag cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat
      721 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      781 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      841 gctcactgcc cgctttcc
//
