LOCUS       sequence-141828-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSTL1-dPSTRr(2,1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Aymoz D, Wosika V, Durandau E, Pelet S
  TITLE     Real-time quantification of protein expression at the single-cell 
            level via dynamic protein synthesis translocation reporters.
  JOURNAL   Nat Commun. 2016 Apr 21;7:11304. doi: 10.1038/ncomms11304.
  PUBMED    27098003
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-21"; volume: "7:11304. doi"; pages: " 
            10.1038/ncomms11304"
COMMENT     Sequence Label: pDA183
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(10..199)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     211..229
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(498..518)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(534..554)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aattaaagcc ttcgagcgtc ccaaaacctt ctcaagcaag gttttcagta taatgttaca
       61 tgcgtacacg cgtctgtaca gaaaaaaaag aaaaatttga aatataaata acgttcttaa
      121 tactaacata actataaaaa aataaatagg gacctagact tcaggttgtc taactccttc
      181 cttttcggtt agagcggatg tggggggagg gcgtgaatgt aagcgtgaca taactaatta
      241 catgactcga ggctagctta ttcttcgatt ttcttacgca gattcgcgat ttctttctcc
      301 aggtacgcga tcaggtcttt tttgtgcagg ttctttttct tcagggtttc gttctcattt
      361 tccagagacg caacttcgtt ttcgagctgc gcaaccaggt tagatctttt aaattccatt
      421 gtaaaaggtg ctgcggcact tccagctttt ttatataagg aagtttgatt gctgccaccg
      481 tcgacactag atccccccac cttccgcttc ttcttgggtt cagcaccagc ccccaccttc
      541 cgcttcttct tgggttcgga tctcataagc tttccctgca ttaaagaatc ggccaaagcc
      601 ctgggggaag ctgtttgagt attaggtgct agggtcgttt ttctctttac caaactaact
      661 ggaagcagcc acgcacctga tttatggtaa ccacctctta gccttagcac aagatgtaag
      721 gtggactcct tctgaatgtt gtaatcagac agcgttctac cgtcttctag ctgcttaccg
      781 gc
//
