LOCUS       sequence-141832-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Dlx6. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Dlx6
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(550..568)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(551..568)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(586..602)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(586..602)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(599..621)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    610..626
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(634..640,641..658,659..664))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    679..700
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(817..834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggccgcggg aattcgatta aacgacaggg atcgaaaacg gggaaatcag gttcaacgga
       61 aaggggaaaa agattcggaa gcctcggacc atttattcca gcctgcagct ccaggcttta
      121 aaccatcgct ttcagcagac tcaatacctg gcccttcccg agagagccga actggctgct
      181 tccttaggac tgacacaaac acaggtgaag atatggtttc agaataagcg ctctaagttt
      241 aagaaattgc tgaagcaggg tagtaaccca cacgagagtg accccctccc gggttcagca
      301 gccctgtcac cacgatcacc agccctgcct ccagtgtggg acgtttctgc ctctgccaag
      361 ggcgtcagta tgcctcccaa cagctacatg ccggggtatt cacactggta ttcctcacca
      421 caccaggaca ccatgcagag accacagatg atgtgacttc tctgagcgaa atcactagtg
      481 aattcgcggc cgcctgcagg tcgaccatat gggagagctc ccaacgcgtt ggatgcatag
      541 cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc
      601 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      661 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      721 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      781 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      841 ggtcgttcgg ctgcggcgag cggtttcagc tcactcaaa
//
