LOCUS       sequence-141846-         545 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo homologous recombination substrate composed of two 
            differentially mutated GFP genes oriented as direct repeats and 
            separated by a drug selection marker .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Pierce AJ, Johnson RD, Thompson LH, Jasin M
  TITLE     XRCC3 promotes homology-directed repair of DNA damage in mammalian 
            cells.
  JOURNAL   Genes Dev. 1999 Oct 15. 13(20):2633-8.
  PUBMED    10541549
REFERENCE   2  (bases 1 to 545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            1999 Oct 15. 13(20):2633-8."
COMMENT     Sequence Label: pDRGFP
FEATURES             Location/Qualifiers
     source          1..545
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(60..79)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    125..180
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(179..198)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat
       61 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
      121 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
      181 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
      241 agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt
      301 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
      361 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
      421 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
      481 gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttta gggatcaaga
      541 tcgcc
//
