LOCUS       sequence-141848-        9328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 9328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP11
FEATURES             Location/Qualifiers
     source          1..9328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1418..1427
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1427..1450
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1463..1489
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1505..1529
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4430..4454)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4467..5034
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4484..5036
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4628..4645)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4855..4874
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5048..5647
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5048..5067)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5544..5564
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5723..6237
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6399..6415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6423..6429,6430..6447,6448..6453))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6468..6489
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6606..6623)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6777..7365)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6857..6876)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7536..8327,8328..8396))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8159..8178
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8397..8501)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8569..8587
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8625..8647)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8747..8766
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8960..8982
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9110..9129
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatctgcc accatggact acaaggatga
     1441 cgatgacaag ctcgatggag gatacccata cgatgttcca gattacgctg ctcgaggtta
     1501 tcaaacaagt ttgtacaaaa aagttggcat ggcagtagcc ccgcgactgt ttggggggct
     1561 ctgcttccgt ttccgggacc agaatccgga agtggctgtt gaggggcgtc ttccaatctc
     1621 gcacagctgc gttggctgta gaagagaacg gacggcgatg gcgacggtcg cagcaaatcc
     1681 agctgctgct gcggcggctg tggcggcggc agcggcggtg actgaggata gagagccaca
     1741 gcacgaggag ctgccaggcc tggacagcca gtggcgccag atagaaaacg gcgagagtgg
     1801 gcgagaacgt ccactgcggg ccggcgaaag ctggttcctt gtggagaagc actggtataa
     1861 gcagtgggag gcatacgtgc agggagggga ccaggactcc agcaccttcc ctggctgcat
     1921 caacaatgcc acactctttc aagatgagat aaactggcgc ctcaaggagg gactggtgga
     1981 aggcgaggat tatgtgctgc tcccagcagc tgcttggcat tacctggtca gctggtatgg
     2041 tctagagcat ggccagccac ccattgaacg caaggtcata gagctgccca acatccagaa
     2101 ggtcgaagtg tacccagtag aactgctgct tgtccggcac aatgatttgg gcaaatctca
     2161 cactgttcag ttcagccata ccgattctat tggcctagta ttgcgcacag ctcgggagcg
     2221 gtttctggtg gagccccagg aagacactcg gctttgggcc aagaactcag aaggctcttt
     2281 ggataggttg tatgacacac acatcacggt tctcgatgcg gcccttgaga ctgggcagtt
     2341 gatcatcatg gagacccgca agaaagatgg cacttggccc agcgcacagc tgcatgtcat
     2401 gaacaacaac atgtcggaag aggatgagga cttcaagggt cagccaggca tctgtggcct
     2461 caccaatctg ggcaacacgt gcttcatgaa ctcggccctg cagtgcctca gcaatgtgcc
     2521 acagctcacc gagtacttcc tcaacaactg ctacctggag gagctcaact tccgcaaccc
     2581 actgggcatg aagggtgaga tcgcagaggc ctatgcagac ctggtgaagc aggcgtggtc
     2641 tggccaccac cgctccattg tgccacatgt gttcaagaac aaggttggcc attttgcatc
     2701 ccaatttctg ggctaccagc agcatgactc tcaggagctg ctgtcattcc tcctggacgg
     2761 gctgcatgag gaccttaatc gggtgaagaa gaaggagtat gtggagctgt gcgatgctgc
     2821 tgggcgaccg gatcaggagg tggcacagga ggcatggcaa aaccacaaac ggcggaacga
     2881 ttctgtgatc gtggacactt tccacggcct cttcaagtcc acgctggtgt gccccgattg
     2941 tggcaatgta tctgtgacct tcgacccctt ctgctacctc agtgttccac tgcctatcag
     3001 ccacaagagg gtcttggagg tcttctttat ccccatggat ccgcgccgca agccagagca
     3061 gcaccggctc gtggtcccca agaaaggcaa gatctcggat ctatgtgtgg ctctgtccaa
     3121 acacacgggc atctcgccag agaggatgat ggtggctgat gtcttcagtc accgcttcta
     3181 taagctctat cagctagagg agcctctgag cagcatcttg gaccgtgatg atatcttcgt
     3241 ctatgaggtg tcaggtcgca ttgaggccat tgagggctca agagaggaca tcgtggttcc
     3301 tgtctacctg cgggagcgca cccctgcccg tgactacaac aactcctact acggcctgat
     3361 gctttttgga caccccctcc tggtatcagt gccccgggac cgcttcacct gggagggcct
     3421 gtataacgtc ctgatgtacc ggctctcacg ctacgtgacc aaacccaact cagatgatga
     3481 ggacgatggg gatgagaaag aagatgacga ggaggataaa gatgacgtcc ctgggccctc
     3541 aactgggggc agcctccgag accctgagcc agagcaggct gggcccagct ctggagtcac
     3601 gaacaggtgc ccgttcctcc tggacaattg ccttggcaca tctcagtggc ccccaaggcg
     3661 acgacgcaag cagctgttca ccctgcagac ggtgaactcc aatgggacca gcgaccgcac
     3721 aacctcccct gaagaagtcc atgcccagcc gtacattgct atcgactggg agccagagat
     3781 gaagaagcgt tactatgacg aggtagaggc tgagggctac gtgaagcatg actgcgtcgg
     3841 gtacgtgatg aagaaggctc ccgtgcggct gcaggagtgc attgagctct tcaccactgt
     3901 ggagaccctg gagaaggaaa acccctggta ctgcccttcc tgcaagcagc accagctggc
     3961 aaccaagaag ctggacctgt ggatgctgcc ggagattctc atcatccacc tgaaacgctt
     4021 ttcctacacc aagttctccc gagagaagct ggacaccctc gtggagtttc ctatccggga
     4081 cctggacttc tctgagtttg tcatccagcc acagaatgag tcgaatccgg agctgtacaa
     4141 atatgacctc atcgcggttt ccaaccatta tgggggcatg cgtgatggac actacacaac
     4201 atttgcctgc aacaaggaca gcggccagtg gcactacttt gatgacaaca gcgtctcccc
     4261 tgtcaatgag aatcagatcg agtccaaggc agcctatgtc ctcttctacc aacgccagga
     4321 cgtggcgcga cgcctgctgt ccccggccgg ctcatctggc gccccagcct cccctgcctg
     4381 cagctcccca cccagctctg agttcatgga tgttaattga ggccggccta cccaactttc
     4441 ttgtacaaag tggttcgata acgaattccg cccccccccc ctaacgttac tggccgaagc
     4501 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     4561 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     4621 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     4681 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     4741 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     4801 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     4861 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     4921 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     4981 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
     5041 cacaaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     5101 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     5161 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     5221 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     5281 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     5341 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     5401 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     5461 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     5521 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     5581 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     5641 tgcctgatgc atcgatgtcg acctgcagcc aagcttatcg ataaaataaa agattttatt
     5701 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta
     5761 agtaacgcca ttttgcaagg catggaaaat acataactga gaatagagaa gttcagatca
     5821 aggttaggaa cagagagaca gcagaatatg ggccaaacag gatatctgtg gtaagcagtt
     5881 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc cctcagcagt
     5941 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     6001 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     6061 ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgata gactgcgtcg
     6121 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     6181 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatggg
     6241 taacagtttc ttgaagttgg agaacaacat tctgagggta ggagtcgaat attaagtaat
     6301 cctgactcaa ttagccactg ttttgaatcc acatactcca atactcctga aatagttcat
     6361 tatggacagc gcagaaagag ctggggagaa ttgtgaaatt gttatccgct cacaattcca
     6421 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6481 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6541 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6601 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6661 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6721 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6781 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6841 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6901 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6961 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7021 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7081 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7141 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7201 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7261 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7321 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7381 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7441 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7501 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7561 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7621 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7681 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7741 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7801 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7861 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7921 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7981 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8041 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8101 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8161 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8221 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8281 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8341 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8401 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8461 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8521 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     8581 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     8641 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     8701 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     8761 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     8821 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     8881 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     8941 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggcgca aggaatggtg
     9001 catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
     9061 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
     9121 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg
     9181 cgtagaggcg attagtccaa tttgttaaag acaggatatc agtggtccag gctctagttt
     9241 tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata aaataaaaga
     9301 ttttatttag tctccagaaa aagggggg
//
