LOCUS       sequence-141852-         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pGPD1-dPSTRy(4,3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Aymoz D, Wosika V, Durandau E, Pelet S
  TITLE     Real-time quantification of protein expression at the single-cell 
            level via dynamic protein synthesis translocation reporters.
  JOURNAL   Nat Commun. 2016 Apr 21;7:11304. doi: 10.1038/ncomms11304.
  PUBMED    27098003
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-21"; volume: "7:11304. doi"; pages: " 
            10.1038/ncomms11304"
COMMENT     Sequence Label: pDA200
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(216..236)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(252..272)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(408..635)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 tgcgccagac ggttgcgcag cgccgctttt tctttacgca gacgcgcgat ttctttttcc
       61 aggtaagcgt tttcattttc gatgaacgca gcgtcattct ccagagtggt aacttcgtta
      121 gatcttttaa attccattgt aaaaggtgct gcggcacttc cagctttttt atataaggaa
      181 gtttgattgc tgccaccgtc gacactagat ccccccacct tccgcttctt cttgggttca
      241 gcaccagccc ccaccttccg cttcttcttg ggttcggatc tcataagctt tccctgcatt
      301 aaagaatcgg ccaaagccct gggggaagct gtttgagtat taggtgctag ggtcgttttt
      361 ctctttacca aactaactgg aagcagccac gcacctgatt tatggtaacc acctcttagc
      421 cttagcacaa gatgtaaggt ggactccttc tgaatgttgt aatcagacag cgttctaccg
      481 tcttctagct gcttaccggc aaagatcaat ctttgttgat ctggagggat accttccttg
      541 tcttgaattt tcgacttaac gttgtcgatg gtatcggaag attcaacttc caatgttatg
      601 gttttaccgg tcaaagtctt gacgaaaatc tgcatgatat cgaattcctg cagcccgggg
//
