LOCUS       sequence-141861-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intermediate cloning vector, with MCS2 containing inducible unstable
            peptide driven by pGPD1 (UbiY 2xNLSs SZ3)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Aymoz D, Wosika V, Durandau E, Pelet S
  TITLE     Real-time quantification of protein expression at the single-cell 
            level via dynamic protein synthesis translocation reporters.
  JOURNAL   Nat Commun. 2016 Apr 21;7:11304. doi: 10.1038/ncomms11304.
  PUBMED    27098003
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-21"; volume: "7:11304. doi"; pages: " 
            10.1038/ncomms11304"
COMMENT     Sequence Label: pDA186
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(217..237)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(253..273)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(409..636)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(662..678)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(662..678)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gtgcgccaga cggttgcgca gcgccgcttt ttctttacgc agacgcgcga tttctttttc
       61 caggtaagcg ttttcatttt cgatgaacgc agcgtcattc tccagagtgg taacttcgtt
      121 agatctttta aattccattg taaaaggtgc tgcggcactt ccagcttttt tatataagga
      181 agtttgattg ctgccaccgt cgacactaga tccccccacc ttccgcttct tcttgggttc
      241 agcaccagcc cccaccttcc gcttcttctt gggttcggat ctcataagct ttccctgcat
      301 taaagaatcg gccaaagccc tgggggaagc tgtttgagta ttaggtgcta gggtcgtttt
      361 tctctttacc aaactaactg gaagcagcca cgcacctgat ttatggtaac cacctcttag
      421 ccttagcaca agatgtaagg tggactcctt ctgaatgttg taatcagaca gcgttctacc
      481 gtcttctagc tgcttaccgg caaagatcaa tctttgttga tctggaggga taccttcctt
      541 gtcttgaatt ttcgacttaa cgttgtcgat ggtatcggaa gattcaactt ccaatgttat
      601 ggttttaccg gtcaaagtct tgacgaaaat ctgcatgata tcgaattcct gcagcccggg
      661 ggatccacta gttctagact ttatattatc aatatttgtg tttgtggagg g
//
