LOCUS       sequence-141868-         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSTL1-dPSTRy(4,3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Aymoz D, Wosika V, Durandau E, Pelet S
  TITLE     Real-time quantification of protein expression at the single-cell 
            level via dynamic protein synthesis translocation reporters.
  JOURNAL   Nat Commun. 2016 Apr 21;7:11304. doi: 10.1038/ncomms11304.
  PUBMED    27098003
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-21"; volume: "7:11304. doi"; pages: " 
            10.1038/ncomms11304"
COMMENT     Sequence Label: pDA199
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(219..239)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(255..275)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(411..638)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(664..680)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(664..680)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttgtgcgcca gacggttgcg cagcgccgct ttttctttac gcagacgcgc gatttctttt
       61 tccaggtaag cgttttcatt ttcgatgaac gcagcgtcat tctccagagt ggtaacttcg
      121 ttagatcttt taaattccat tgtaaaaggt gctgcggcac ttccagcttt tttatataag
      181 gaagtttgat tgctgccacc gtcgacacta gatcccccca ccttccgctt cttcttgggt
      241 tcagcaccag cccccacctt ccgcttcttc ttgggttcgg atctcataag ctttccctgc
      301 attaaagaat cggccaaagc cctgggggaa gctgtttgag tattaggtgc tagggtcgtt
      361 tttctcttta ccaaactaac tggaagcagc cacgcacctg atttatggta accacctctt
      421 agccttagca caagatgtaa ggtggactcc ttctgaatgt tgtaatcaga cagcgttcta
      481 ccgtcttcta gctgcttacc ggcaaagatc aatctttgtt gatctggagg gataccttcc
      541 ttgtcttgaa ttttcgactt aacgttgtcg atggtatcgg aagattcaac ttccaatgtt
      601 atggttttac cggtcaaagt cttgacgaaa atctgcatga tatcgaattc ctgcagcccg
      661 ggggatccac tagttctaga ggtctaaaac tttctatgtt ctatttttct atttt
//
