LOCUS       sequence-141880-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 hypothetical acetic acid resistance 
            protein B, cleavable N-terminal NusA fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 acetic acid resistance (aar) gene 
            constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK122
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(124..141)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(148..180)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             complement(771..785)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(804..821)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(837..881)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(891..908)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taccatcggc gctacggcgt ttcacttctg agttcggcat ggggtcaggt gggaccaccg
       61 cgctactgcc gccaggcaaa ttctgtttta tcagcctagg aacgcccaac ttaattaaca
      121 ttagtggtgg tggtggtggt gctcgagatc ctctgggtct tcaggagcga gttctggctg
      181 gcttgcacgt gtatacagct gtgcggccgc aagcttcgaa cgcgtatcga tggtaccgtc
      241 gacgtcctgc aggcgcgcct gtacagaatt cggatccacg agctcccgga ctctgtacag
      301 aattctcaag cacgcagtgc cagcggcgga acagcggagt gagcgctggc cggcagtgca
      361 cggcactgcg cgcggtacca tttgatgtac ccgggcagtg cgcataccgc agattccggc
      421 agacgttgcg gcggcaccgg cataccgcct tcccatgcac ggatgttatg caggtatttg
      481 cgacgccatg cctgcagttt agtcggcgcg aactcagcac gttcacgatt cagaccacac
      541 agcgcggacg gaggtgcata gccacggtat ttacgagccg gtgcgtcggt caggcacgct
      601 ggccagtgag ctggatccgg aatgcgttcg agacgctgct gctgcttctg gaaggtacgg
      661 taacgaaagg tggccagata cagaacagat gcgaaagtac ggtgaccgaa acatgcgttc
      721 gcacacatgc gagcaacgcg cagaccaaag aactgcggtt tacccagcat tttgtcgtcg
      781 tcatcaatcg taccagaacc cgcgctgccc cgggggacca gaccagttgg cggtggcgag
      841 tccatgtgct ggcgttcaaa tttcgcagca gcggtttctt tacccgcgga gtgatggtga
      901 tggtgatgac cagaaccact agtcgcttcg tcaccgaacc agcaaatatt acgggcagcc
      961 ataatcagtg ctccggcttt ttcgtcggtc aacccttcga tatcagcc
//
