LOCUS       sequence-141885-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express c-Myc-tagged human NLRP3(R260W) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Dorfleutner A, Bryan NB, Talbott SJ, Funya KN, Rellick SL, Reed JC, 
            Shi X, Rojanasakul Y, Flynn DC, Stehlik C
  TITLE     Cellular pyrin domain-only protein 2 is a candidate regulator of 
            inflammasome activation.
  JOURNAL   Infect Immun. 2007 Mar;75(3):1484-92. Epub 2006 Dec 18.
  PUBMED    17178784
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2007-03"; volume: "75(3)"; pages: "1484-92. Epub 
            2006 Dec 18"
COMMENT     Sequence Label: pcDNA3-Myc-NLRP3(R260W)
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     60..79
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             106..135
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctatataagc agagctctct ggctaactga gaacccactg cttactggct tatcgaaatt
       61 aatacgactc actataggga gacccaagct tcgatcgtcg acatggaaca gaaactcatc
      121 tctgaagagg atctgtctag aggatccgag atgaagatgg caagcacccg ctgcaagctg
      181 gccaggtacc tggaggacct ggaggatgtg gacttgaaga aatttaagat gcacttagag
      241 gactatcctc cccagaaggg ctgcatcccc ctcccgaggg gtcagacaga gaaggcagac
      301 catgtggatc tagccacgct aatgatcgac ttcaatgggg aggagaaggc gtgggccatg
      361 gccgtgtgga tcttcgctgc gatcaacagg agagaccttt atgagaaagc aaaaagagat
      421 gagccgaagt ggggttcaga taatgcacgt gtttcgaatc ccactgtgat atgccaggaa
      481 gacagcattg aagaggagtg gatgggttta ctggagtacc tttcgagaat ctctatttgt
      541 aaaatgaaga aagattaccg taagaagtac agaaagtacg tgagaagcag attccagtgc
      601 attgaagaca ggaatgcccg tctgggtgag agtgtgagcc tcaacaaacg ctacacacga
      661 ctgcgtctca tcaaggagca ccggagccag caggagaggg agcaggagct tctggccatc
      721 ggcaagacca agacgtgtga gagccccgtg agtcccatta agatggagtt gctgtttgac
      781 cccgatgatg agcattctga gcctgtgcac accgtggtgt tccagggggc ggcagggatt
      841 gggaaaacaa tcctggccag gaagatgatg ttggactggg catcggggac actctaccaa
      901 gacaggtttg actatctgtt ctatatccac tgttgggagg tgagccttgt gac
//
