LOCUS       sequence-141890-        1085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human ASC with a C-terminal EGFP tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   de Almeida L, Khare S, Misharin AV, Patel R, Ratsimandresy RA, 
            Wallin MC, Perlman H, Greaves DR, Hoffman HM, Dorfleutner A, Stehlik
            C
  TITLE     The PYRIN Domain-only Protein POP1 Inhibits Inflammasome Assembly 
            and Ameliorates Inflammatory Disease.
  JOURNAL   Immunity. 2015 Aug 18;43(2):264-76. doi: 
            10.1016/j.immuni.2015.07.018. Epub 2015 Aug 11.
  PUBMED    26275995
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2015.07.018"; journalName: "Immunity"; date: 
            "2015-08-18- 18"; volume: "43"; issue: "2"; pages: "264-76"
COMMENT     Sequence Label: pLEX-MCS-ASC-GFP
FEATURES             Location/Qualifiers
     source          1..1085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      776..785
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(827..848)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgactcta ctagaggatc gactagcgct accggactca
      121 gatctcgagc tcaagcttcg aattccctgg agccatgggg cgcgcgcgcg acgccatcct
      181 ggatgcgctg gagaacctga ccgccgagga gctcaagaag ttcaagctga agctgctgtc
      241 ggtgccgctg cgcgagggct acgggcgcat cccgcggggc gcgctgctgt ccatggacgc
      301 cttggacctc accgacaagc tggtcagctt ctacctggag acctacggcg ccgagctcac
      361 cgctaacgtg ctgcgcgaca tgggcctgca ggagatggcc gggcagctgc aggcggccac
      421 gcaccagggc tctggagccg cgccagctgg gatccaggcc cctcctcagt cggcagccaa
      481 gccaggcctg cactttatag accagcaccg ggctgcgctt atcgcgaggg tcacaaacgt
      541 tgagtggctg ctggatgctc tgtacgggaa ggtcctgacg gatgagcagt accaggcagt
      601 gcgggccgag cccaccaacc caagcaagat gcggaagctc ttcagtttca caccagcctg
      661 gaactggacc tgcaaggact tgctcctcca ggccctaagg gagtcccagt cctacctggt
      721 ggaggacctg gagcggagct gcggcctcga cggtaccgcg ggcccgggat ccatcgccac
      781 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      841 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      901 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      961 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     1021 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     1081 cttca
//
