LOCUS       sequence-141894-         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human ASC with a C-terminal EGFP tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   de Almeida L, Khare S, Misharin AV, Patel R, Ratsimandresy RA, 
            Wallin MC, Perlman H, Greaves DR, Hoffman HM, Dorfleutner A, Stehlik
            C
  TITLE     The PYRIN Domain-only Protein POP1 Inhibits Inflammasome Assembly 
            and Ameliorates Inflammatory Disease.
  JOURNAL   Immunity. 2015 Aug 18;43(2):264-76. doi: 
            10.1016/j.immuni.2015.07.018. Epub 2015 Aug 11.
  PUBMED    26275995
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2015.07.018"; journalName: "Immunity"; date: 
            "2015-08-18- 18"; volume: "43"; issue: "2"; pages: "264-76"
COMMENT     Sequence Label: pLEX-MCS-ASC-GFP
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(248..269)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     597..616
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     856..877
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctcgtcaaga agacagggcc aggtttccgg gccctcacat tgccaaaaga cggcaatatg
       61 gtggaaaata acatatagac aaacgcacac cggccttatt ccaagcggct tcggccagta
      121 acgttagggg ggggggaggg agaggggcga ccggtggcca gacgcgtcgg gccctctaga
      181 ctcgagtgcg catgcggccg ctttacttgt acagctcgtc catgccgaga gtgatcccgg
      241 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
      301 gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
      361 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
      421 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
      481 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
      541 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
      601 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
      661 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
      721 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
      781 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
      841 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc cc
//
