LOCUS       sequence-141896-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing iBlueberry and mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Yu D, Dong Z, Gustafson WC, Ruiz-Gonzalez R, Signor L, Marzocca F, 
            Borel F, Klassen MP, Makhijani K, Royant A, Jan YN, Weiss WA, Guo S,
            Shu X
  TITLE     Rational design of a monomeric and photostable far-red fluorescent 
            protein for fluorescence imaging in vivo.
  JOURNAL   Protein Sci. 2016 Feb;25(2):308-15. doi: 10.1002/pro.2843. Epub 2015
            Dec 31.
  PUBMED    26549191
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2843"; 
            journalName: "Protein Sci"; date: "2016-02"; volume: "25"; issue: 
            "2"; pages: "308-15"
COMMENT     Sequence Label: pcDNA3.1_LifeAct-iBlueberry
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..182)
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      179..188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(223..242)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(224..242)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(287..490)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(316..336)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        491..870
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccggctctt tcacagccag caggataccg tgcggctgaa tggagcccgc caggtggatc
       61 tgctcgcgtt cacagttagc cagaaacgcg tggccgaatg ctgaggtagt cagcggtacc
      121 gacatgctgc cctcctcctt ggagatggac tcgaacttct tgatcaagtc ggccacgccc
      181 atggtggcaa gcttaagttt aaacgctagc cagcttgggt ctccctatag tgagtcgtat
      241 taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat
      301 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
      361 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
      421 ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa
      481 accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
      541 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
      601 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
      661 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
      721 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
      781 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
      841 actattaata actagtcaat aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa
      901 gcagcgcaaa acgcctaacc ctaagcagat
//
