LOCUS       sequence-141898-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human ASC with a C-terminal EGFP tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   de Almeida L, Khare S, Misharin AV, Patel R, Ratsimandresy RA, 
            Wallin MC, Perlman H, Greaves DR, Hoffman HM, Dorfleutner A, Stehlik
            C
  TITLE     The PYRIN Domain-only Protein POP1 Inhibits Inflammasome Assembly 
            and Ameliorates Inflammatory Disease.
  JOURNAL   Immunity. 2015 Aug 18;43(2):264-76. doi: 
            10.1016/j.immuni.2015.07.018. Epub 2015 Aug 11.
  PUBMED    26275995
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2015.07.018"; journalName: "Immunity"; date: 
            "2015-08-18- 18"; volume: "43"; issue: "2"; pages: "264-76"
COMMENT     Sequence Label: pLEX-MCS-ASC-GFP
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(775..799)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(796..999)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(825..845)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggtgaacagc tcctcgccct tgctcaccat ggtggcgatg gatcccgggc ccgcggtacc
       61 gtcgaggccg cagctccgct ccaggtcctc caccaggtag gactgggact cccttagggc
      121 ctggaggagc aagtccttgc aggtccagtt ccaggctggt gtgaaactga agagcttccg
      181 catcttgctt gggttggtgg gctcggcccg cactgcctgg tactgctcat ccgtcaggac
      241 cttcccgtac agagcatcca gcagccactc aacgtttgtg accctcgcga taagcgcagc
      301 ccggtgctgg tctataaagt gcaggcctgg cttggctgcc gactgaggag gggcctggat
      361 cccagctggc gcggctccag agccctggtg cgtggccgcc tgcagctgcc cggccatctc
      421 ctgcaggccc atgtcgcgca gcacgttagc ggtgagctcg gcgccgtagg tctccaggta
      481 gaagctgacc agcttgtcgg tgaggtccaa ggcgtccatg gacagcagcg cgccccgcgg
      541 gatgcgcccg tagccctcgc gcagcggcac cgacagcagc ttcagcttga acttcttgag
      601 ctcctcggcg gtcaggttct ccagcgcatc caggatggcg tcgcgcgcgc gccccatggc
      661 tccagggaat tcgaagcttg agctcgagat ctgagtccgg tagcgctagt cgatcctcta
      721 gtagagtcgg tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg
      781 acggttcact aaacgagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
      841 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
      901 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
      961 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     1021 tacgtagatg tactgcca
//
