LOCUS       sequence-141900-         698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto 
            ACP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Worthington AS, Burkart MD
  TITLE     One-pot chemo-enzymatic synthesis of reporter-modified proteins.
  JOURNAL   Org Biomol Chem. 2006 Jan 7;4(1):44-6. Epub 2005 Nov 24.
  PUBMED    16357994
REFERENCE   2  (bases 1 to 698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Org Biomol 
            Chem."; date: "2006-01-7"; volume: "4(1)"; pages: "44-6. Epub 2005 
            Nov 24"
COMMENT     Sequence Label: Sfp pet29b C-terminal His Tag
FEATURES             Location/Qualifiers
     source          1..698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             678..695
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgaagattta cggaatttat atggaccgcc cgctttcaca ggaagaaaat gaacggttca
       61 tgactttcat atcacctgaa aaacgggaga aatgccggag attttatcat aaagaagatg
      121 ctcaccgcac cctgctggga gatgtgctcg ttcgctcagt cataagcagg cagtatcagt
      181 tggacaaatc cgatatccgc tttagcacgc aggaatacgg gaagccgtgc atccctgatc
      241 ttcccgacgc tcatttcaac atttctcact ccggccgctg ggtcattggt gcgtttgatt
      301 cacagccgat cggcatagat atcgaaaaaa cgaaaccgat cagccttgag atcgccaagc
      361 gcttcttttc aaaaacagag tacagcgacc ttttagcaaa agacaaggac gagcagacag
      421 actattttta tcatctatgg tcaatgaaag aaagctttat caaacaggaa ggcaaaggct
      481 tatcgcttcc gcttgattcc ttttcagtgc gcctgcatca ggacggacaa gtatccattg
      541 agcttccgga cagccattcc ccatgctata tcaaaacgta tgaggtcgat cccggctaca
      601 aaatggctgt atgcgccgca caccctgatt tccccgagga tatcacaatg gtctcgtacg
      661 aagagctttt actcgagcac caccaccacc accactga
//
