LOCUS       sequence-141901-         258 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the acyl carrier protein (ACP) from the E. coli fatty acid
            synthase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Worthington AS, Rivera H, Torpey JW, Alexander MD, Burkart MD
  TITLE     Mechanism-based protein cross-linking probes to investigate carrier 
            protein-mediated biosynthesis.
  JOURNAL   ACS Chem Biol. 2006 Dec 20;1(11):687-91.
  PUBMED    17184132
REFERENCE   2  (bases 1 to 258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol."; date: "2006-12-20"; volume: "1(11)"; pages: "687-91"
COMMENT     Sequence Label: AcpP pet29a C-terminal His Tag
FEATURES             Location/Qualifiers
     source          1..258
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..231
                     /codon_start=1
                     /gene="acpP"
                     /product="acyl carrier protein (ACP) from E. coli"
                     /label=ACP
                     /translation="STIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELVMA
                     LEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     CDS             238..255
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcactatcg aagaacgcgt taagaaaatt atcggcgaac agctgggcgt taagcaggaa
       61 gaagttacca acaatgcttc tttcgttgaa gacctgggcg cggattctct tgacaccgtt
      121 gagctggtaa tggctctgga agaagagttt gatactgaga ttccggacga agaagctgag
      181 aaaatcacca ccgttcaggc tgccattgat tacatcaacg gccaccaggc gctcgagcac
      241 caccaccacc accactga
//
