LOCUS       sequence-141904-        1301 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the ketosynthase FabF/KASII from the E. coli fatty acid 
            synthase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Worthington AS, Rivera H, Torpey JW, Alexander MD, Burkart MD
  TITLE     Mechanism-based protein cross-linking probes to investigate carrier 
            protein-mediated biosynthesis.
  JOURNAL   ACS Chem Biol. 2006 Dec 20;1(11):687-91.
  PUBMED    17184132
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol."; date: "2006-12-20"; volume: "1(11)"; pages: "687-91"
COMMENT     Sequence Label: FabF (KASII) pet28 N-terminal His Tag
FEATURES             Location/Qualifiers
     source          1..1301
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..29
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             36..53
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tgaaacacca ccaccaccac caccaccacg gtggtctggt tccgcgtggt tcccatggcg
       61 tgtctaagcg tcgtgtagtt gtgaccggac tgggcatgtt gtctcctgtc ggcaataccg
      121 tagagtctac ctggaaagct ctgcttgccg gtcagagtgg catcagccta atcgaccatt
      181 tcgatactag cgcctatgca acgaaatttg ctggcttagt aaaggatttt aactgtgagg
      241 acattatctc gcgcaaagaa cagcgcaaga tggatgcctt cattcaatat ggaattgtcg
      301 ctggcgttca ggccatgcag gattctggcc ttgaaataac ggaagagaac gcaacccgca
      361 ttggtgccgc aattggctcc gggattggcg gcctcggact gatcgaagaa aaccacacat
      421 ctctgatgaa cggtggtcca cgtaagatca gcccattctt cgttccgtca acgattgtga
      481 acatggtggc aggtcatctg actatcatgt atggcctgcg tggcccgagc atctctatcg
      541 cgactgcctg tacttccggc gtgcacaaca ttggccatgc tgcgcgtatt atcgcgtatg
      601 gcgatgctga cgtgatggtt gcaggtggcg cagagaaagc cagtacgccg ctgggcgttg
      661 gtggttttgg cgcggcacgt gcattatcta cccgcaatga taacccgcaa gcggcgagcc
      721 gcccgtggga taaagagcgt gatggtttcg tactgggcga tggtgccggt atgctggtac
      781 ttgaagagta cgaacacgcg aaaaaacgcg gtgcgaaaat ttacgctgaa ctcgtcggct
      841 ttggtatgag cagcgatgct tatcatatga cgtcaccgcc agaaaatggc gcaggcgcag
      901 ctctggcgat ggcaaatgct ctgcgtgatg caggcattga agcgagtcag attggctacg
      961 ttaacgcgca cggtacttct acgccggctg gcgataaagc tgaagcgcag gcggtgaaaa
     1021 ccatcttcgg tgaagctgca agccgtgtgt tggtaagctc cacgaaatct atgaccggtc
     1081 acctgttagg tgcggcgggt gcagtagaat ctatctactc catcctggcg ctgcgcgatc
     1141 aggctgttcc gccaaccatc aacctggata acccggatga aggttgcgat ctggatttcg
     1201 taccgcacga agcgcgtcag gttagcggaa tggaatacac tctgtgtaac tccttcggct
     1261 tcggtggcac taatggttct ttgatcttta aaaagatcta a
//
