LOCUS       sequence-141914-        4619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used to drive expression in zebrafish microglia..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: p5E-mpeg1.1
FEATURES             Location/Qualifiers
     source          1..4619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        690..708
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2593..2609)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2593..2609)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(2646..2664)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2665..2788
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2861..2879)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2884..2900)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2884..2900)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3002..3021)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3013..3822
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3080..3099)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3969..4557
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4458..4477
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttggt aatacgactc actatagggc gaattgggta
      721 ccgggccccc cctcgagtgt tggagcacat ctgacatctg aaattatttg aaagcagcag
      781 aaatgttttt acagctatta caaaatttac ttaacaagaa tcctttaaaa tatgacaatt
      841 tattgccaat aatttacagt aagttttgca gtactttaat attacaaatt atacaactgt
      901 gctggcacac aaaaaataac ttaatccagc acaacaacaa caacaacaac tacaaaaagc
      961 atatttcctt ttgctttttc ttgttcactc aacttttgga aacatcaact gcactgacct
     1021 aaaattatac agtatgtcag taatacaaaa acacttagtt aggttaacat aagattatta
     1081 gttttctgga gaggtgaact actctgtaag aaaccttttt aagttgtgcc aactaaacat
     1141 ttttatcagc agaactacaa tgcttgatga gtgaacaaaa aaacaaacaa accttatttc
     1201 cttttgtttt ttcttgtgta cttaacattt ttttcacctt tatttgaaac gacagtaggg
     1261 agtatggaca ggaaagtgtt gggagaggag agggatgagc atagaaccag gaggtaggaa
     1321 tcaaactcag gtcactatga acaccggagt gccaggtgtc aacacactaa ccactacacc
     1381 atcaactcca acatttactt gaaaacatca gttgagtgaa caaaaaactc ttgtggaaat
     1441 tggtactgag aaataataca ttatcacaac aaacaaaaca gagacaaatg ttaacaaaac
     1501 aattattaga aacagaatta gaaaccggac aggaggtaaa acaataatga taaacaagac
     1561 aaggatcaaa acatggcagg acaaagacag ttttttgttc actcaacctc agatatttta
     1621 ggtcagtgca actgatgttt acaaaggttg tatgcatgtc aacaccaatg gtgtagtgat
     1681 tagtgtgttg acacaaggca ctccagtgtt cacactgatg ctggtttgat tcccacatcg
     1741 atgtcctttg ctaatcctgc ctctctcccc atgctttcct gtctatactc tctactgttg
     1801 tgtcaattaa aggtgaaaaa agttaagcac aaaagaaaaa tctaaggata taaggattat
     1861 gttttttttt gttcactcaa aatcaggcat tccagttctg cagacaaaaa atgttcagtt
     1921 ggcacaactt aaaaagtttt gttacagagt agttcacctc tccagaaaac taataatctt
     1981 atgttaaccc aactaaatgt ttttactgaa aaataattga atgtcagtgc agctgatgtt
     2041 tttttaaaag tggagagaac aaaaaaagtg aaaggaaata aggattcatt gttgtaccga
     2101 cttatttttt tgaagaaaat tctgctatag ggctttcatg atcccctatg ctcacagcag
     2161 ttttaggaga tgcttataat aaagaggatt tgaaaaacct gctaatcttg tacatttcta
     2221 aaacaagaag ccaaccaatt tcaattcctc ctctttcaaa gccaatggaa aatttaaaac
     2281 gcacagtctc ttgcgtcatc aaaaccattc tgaagactca tcaaaatcct tcgtagcttt
     2341 aaatataaat ctattattga acagatcata cttcacaaaa aatataaata tatttgattc
     2401 attcacttca tgaaattgtc cttcaactta acgtttgaac gcaaggtacc aaaacacaaa
     2461 gaggaacctc atagtctcat gacccttccc ccacggattt gaaactgaga agtggtttta
     2521 ttgaggaact cagcaaaatc tataatcacc atatttgatt gaaaatcaca ttagtgcagg
     2581 agacagcaaa aggatccact agttctagag cggccgccac cgcggtggag ctccagcttt
     2641 tgttcccttt agtgagggtt aattcaagtt tgtacaaaaa agttgaacga gaaacgtaaa
     2701 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     2761 gtaaaacaca acatatgcag tcactatgaa ccaactactt agatggtatt agtgacctgt
     2821 agaattcgag ctctagagct gcagggcggc cgcgatatcc cctatagtga gtcgtattac
     2881 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     2941 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     3001 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     3061 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     3121 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     3181 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     3241 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     3301 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     3361 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     3421 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     3481 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     3541 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     3601 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     3661 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     3721 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     3781 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     3841 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     3901 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3961 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4021 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4081 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     4141 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4201 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4261 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4321 cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt
     4381 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4441 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4501 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4561 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtt
//
