LOCUS       sequence-141917-       10413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10413)
  TITLE     Boxem lab CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJJR81
FEATURES             Location/Qualifiers
     source          1..10413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             661..966
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             1839..1859
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(1877..1910)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1920..1949
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3766..4089)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6007..6031
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6034..7059
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6034..7059
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7060..7084)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7437..7470)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7489..7512
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7516..7539
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7543..7566
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(7614..7919)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8192..8208)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8192..8208)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8205..8227)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8216..8232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8240..8246,8247..8264,8265..8270))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8285..8306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8423..8440)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8594..9182)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8674..8693)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9353..10144,10145..10213))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9976..9995
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10214..10318)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10386..10404
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtta cccgaagtat
      421 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
      481 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga
      541 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
      601 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa
      661 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
      721 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      781 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      841 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      901 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      961 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg
     1021 gagcctcagg agcatcgatg tccgaactca tcaaggagaa catgcacatg aagctctaca
     1081 tggagggaac cgtagacaac caccacttca agtgcacctc cgagggagag ggaaagccat
     1141 acgagggaac ccaaaccatg cgtatcaagg tcgtcgaggg aggaccactc ccattcgcct
     1201 tcgacatcct cgccacctcc ttcctctacg ggtccaaggt aagtttaaac atatatatac
     1261 taactaaccc tgattattta aattttcaga ccttcatcaa ccacacccaa ggaatcccag
     1321 acttcttcaa gcaatccttc ccagagggat tcacctggga gcgtgtcacc acctacgagg
     1381 acggaggagt cctcaccgcc acccaagaca cctccctcca agacggatgc ctcatctaca
     1441 acgtcaagat ccgtggagtc aacttcacct ccaacggacc agtcatgcaa aagaagaccc
     1501 tcggatggga gaccttcacc gagaccctct acccagccga cggaggacta gagggacgta
     1561 acgacatggc cctcaagctc gtcggaggct cccacctcat cgccaacgcc aaggtaagtt
     1621 taaacagttc ggtactaact aaccatacat atttaaattt tcagaccacc taccgttcca
     1681 agaagccagc caagaacctc aagatgccag gagtctacta cgttgactac cgtctagagc
     1741 gtatcaagga ggccaacaac gagacctacg tcgagcaaca cgaggtcgcc gtcgcccgtt
     1801 actgcgacct cccatccaag ctcggacaca agctcaacga gaatctgtac tttcaatccg
     1861 gaaaggtaag tttaaaataa cttcgtatag catacattat acgaagttat tttcagggag
     1921 agcaaaagct tatctccgag gaggacctct aaggatgatc gacgccaacg tcgttgaatt
     1981 ttcaaatttt aaatactgaa tatttgtttt ttttcctatt atttatttat tctctttgtg
     2041 ttttttttct tgctttctaa aaaattaatt caatccaaat ctaaacattt ttttttctct
     2101 ttccgtctcc caattcgtat tccgctcctc tcatctgaac acaatgtgca agtttattta
     2161 tcttctcgct ttcatttcat taggacgtgg ggggaattgg tggaaggggg aaacacacaa
     2221 aaggatgatg gaaatgaaat aaggacacac aatatgcaac aacattcaat tcagaaatat
     2281 ggaggaaggt ttaaaagaaa acataaaaat atatagagga ggaaggaaac tagctgccct
     2341 cgattgtcta tcttccattc tcaccaagtt tcaacaactt tattcagtca ttcttctctg
     2401 gttcatttcc caaccaatct tcccgaataa agcccatctg tcctttcggc cacattcctc
     2461 acctacatcc gtactcctta tctcccgttc ttatccagtc ttttatcgtc tctgcgtctc
     2521 gcacactgtc tcgtcggtaa tgtaaaaaaa aaacaaaaaa aacttgaaaa cttgcggaaa
     2581 gatttcctaa taaaaggaga cgaatccggt aggaaatcga caagtctctg cttctcgaag
     2641 actaggtgag ttacatttct ttggccgaat tattttgaaa tcctaaattt gataatttca
     2701 gagatgtctg taaaacttgc gtgttatgtg acggcttcgg tcactgtcgc cactcttatg
     2761 gtatgcttca tgaccatgtc aaccatctac tcggaggttg atggattcag agagaagctt
     2821 gataccgaga tgaatgtgtt cagagtgagt taaattatat tttgaatttt aaataatttt
     2881 taattttcta gcaatctacc aatggattgt ggaaggacat agttgtcatc ggaagatcta
     2941 gcaagcgtgt ccgttgtcaa tatgaagaga ccaacgctac cccaactcca catgctgatg
     3001 gatccccatc tgctccacca ggtcaaccac cagcagttcc accagtcttc aaccagccaa
     3061 agactccaaa tggagccaat ggaaatggac caacctgcaa ctgcaatgct gataacaagt
     3121 gcccagctgg accatccgga ccaaagggag ttccaggagt tccaggactc gacggagttc
     3181 caggacttga cggtgttcca ggagttggag ctgatgatat cgctccacaa cgcgagtctg
     3241 tcggatgctt cacttgccca caaggaccag ttggaccacc aggagctctt ggaagaccag
     3301 gaccacgtgg acttccagga ccaagaggac aaaatggaaa cccaggaaga gatggacaac
     3361 caggacatcc aggagagcaa ggatcatccg gtcaaatcgg aaagatcgga gagccaggac
     3421 caccaggaga gaagggacgc gacgccgagc atccaatcgg aagaccagga ccaaagggac
     3481 caagaggaga tcaaggacca acaggaccag ctggacagaa cggtcttcac ggaccaccag
     3541 gagagccagg aaccgttgga ccagaaggac catctggaaa gcaaggacgt caaggaccag
     3601 acggaaccca gggagagact ggaccagacg gaagaccagg aaaggatgcc gagtactgcc
     3661 agtgcccaga caagtctcca ccatcagagg ctgtcaacgc caaccgtgga tacagaaata
     3721 tctaaattgt tggtgttttc taataaaata tttgagattc agtgagactt ttttcttggc
     3781 ggcacaataa agtttcctct ttttgttggg tcactcaacg aacacaatac aaatatttga
     3841 gcgctcgcat ttttctcaac ctttcgggtt taaagcactt caaaatggaa ttgaagagag
     3901 attgtgaaag tacatcagac agaatcacat tttgatagca ttcacttcac tcagatgcaa
     3961 acttttcagt gactataaaa agtcataaaa taataacatt aaaaaagcaa ataaattagc
     4021 gagagaattt tttgacaaaa agaaagaaga gtgatagaga agaagggaat gcttgaaagg
     4081 attttgcatt tatcttaatc accgtcttcg agaagacgaa ccatggcgcc agtttcagaa
     4141 tccaagtttc taatatagtt catcacaatg ttgacgttgg tccaacctcc ggcttgcata
     4201 atctctggga tggaaactcc agcgcgagcc atgtcacggg cagctccgac acgggcggaa
     4261 tgtccagacc aagccaagta acgttgtccg gaatcatcct tggctccgta gatgagacgg
     4321 tgggtagctt cgaagattcc tgaaaattta ttttcagctt attcttagct aatatgttgt
     4381 ttaaacttac cttcaagagc tcgagtcgag agttgtgatg ttgcactcgg ggcagcaact
     4441 ccgttcttgc gaacacgaca gaacaggtag ttgtttggat catcggcgac tccggaaacc
     4501 gaaatccagc gctcaacgag cttggtaact ccaagggaga gcgctttttc gactccggca
     4561 gtcgagacca gcgtttttgt acgtccgatg tggatgagca ttcttcctcc gtctgtgcga
     4621 ctaatatcct tgacgcgaat gcgggcaatt tcggcgatac ggagaagtgt gttgtaagca
     4681 attccgagga aggcgaggtt tcggatgtcc tggcaacgat cggaattttc cataagagaa
     4741 cggacctgat cgaagtcggt tctgaaaatt tcgtcaagtc ttaggattga atacattaat
     4801 atttaaactt acctttcgaa ggcgagggct tgcttggctc tctctccagc atcaacattt
     4861 tccttgcgga tacgtctcat tacgagagat acggcattag aatctgatgg gcgtggaagt
     4921 ccagatcggc gatggagcat attgagctgt ccgagatgtt gttggatcgt cttcaccgcc
     4981 aatccacgag cttggaggta gagcaagtag tcacgaacgt cttctggctc ggctgggaac
     5041 cacttgcggt tgttaagctt gcaccaggca gcccaagaac ggcacactga gagaagcatc
     5101 ttccaggtgt gctcggagaa ggcttgtctg tcacggaaca tatccatgag gttcttgcga
     5161 acttcatcac ttgtcgcgtc aactggaagt gctggaagat tttgatgaac agtgagaaga
     5221 tttgacatat tttcgaagtt ttttagatgc actagaacaa agcgtgttgg cttcctctga
     5281 gcccgctttc cttatatacc cgcattctgc agccttacag aatgttctag aaggtcctag
     5341 atgcattcgt ttgaaaatac tcccggtggg tgcaaagaga cgcagacgga aaatgtatct
     5401 gggtctcttt attgtgtaca ctacttttcc atgtaccgaa tgtgagtcgc cctccttttg
     5461 caacaagcag ctcgaatgtt ctagaaaaag gtggaaaata gtataaatac cgttgaaaat
     5521 aaataccgaa caacatttgc tctaattgtg aaattagaaa tcttcaaact ataatcatgt
     5581 cactttacca ctatttccgt ccagctcaac gttccgtttt tggtgatcat ttttgctttc
     5641 gtcgtaaatc tacacacgcg tctcttccgt gcgagagtcc aagccagcag ccaaattcgt
     5701 tgactgagta ttcaacgttt atacgttgtc ggcaacgaga aataggaaaa tgcatcggga
     5761 aatgttcttt tttcgatttt ttccaaggtt ttgacaaatt ttaccacgaa ttttgctatg
     5821 ttttcaatta aaaaatatgt tattcaactg tttctatgag gaaaataagg ctttgcatgt
     5881 aattttctta ttcagcataa tttttaatta atttgaattt tctgtcctaa cgtttatttt
     5941 gttttcttgg ttatgactga tctgaaatta atttttgaat tttaaggtaa tatgtcaggc
     6001 ggtgccgcaa gtttgtacaa aaaagcaggc tccatgaaaa agcctgaact caccgcgacg
     6061 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     6121 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     6181 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     6241 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     6301 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     6361 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     6421 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     6481 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     6541 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     6601 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     6661 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     6721 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     6781 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     6841 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     6901 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     6961 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     7021 aaccgacgcc ccagcactcg tccgagggca aaggaataga cccagctttc ttgtacaaag
     7081 tgggtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta
     7141 tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc ttttaagtca
     7201 cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata aaaagtcgaa
     7261 aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta cacaatgttc
     7321 tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc atagaaaaaa
     7381 ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa tttttaataa
     7441 cttcgtatag catacattat acgaagttat tttcagggag ccggatctga ttataaagac
     7501 gatgacgata agcgtgacta caaggacgac gacgacaagc gtgattacaa ggatgacgat
     7561 gacaagagac tagttcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt
     7621 ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc
     7681 acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag
     7741 ctttcatccc cgatatgcac caccgggtaa agttcacggg ggactttatc tgacagcaga
     7801 cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt
     7861 acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt
     7921 tcaccagccc ctgttctcgt cggcaaaaga gccgttcatt tcaataaacc gggcgacctc
     7981 agccatccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt
     8041 ctgcatggtt gtgcttaccg aaccggagat attgacatca tatatgcctt gagcaactga
     8101 tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcatacct ctttttgaca
     8161 tacttcgggt tcctaggaaa tcgataacat ggtcatagct gtttcctgtg tgaaattgtt
     8221 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8281 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8341 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8401 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8461 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8521 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8581 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8641 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8701 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8761 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8821 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8881 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8941 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9001 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     9061 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9121 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9181 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9241 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9301 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9361 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9421 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9481 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9541 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9601 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9661 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9721 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9781 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9841 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9901 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9961 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10021 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10081 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10141 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10201 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10261 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10321 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
    10381 ataaaaatag gcgtatcacg aggccctttc gtc
//
