LOCUS       sequence-141922-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSIREN-RetroQ vector containing shRNA sequence to BPTF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  TITLE     BPTF shRNA Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSIREN-RetroQ-BPTF-sh27
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(75..323)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(133..152)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(303..323)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(363..379)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(395..417)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tacccggtag aattcacgcg taaaaaaagg gaagtatcga gtctactctc ttgaagtaga
       61 ctcgatactt ccctggatcc tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa
      121 tactttcaag ttacggtaag catatgatag tccattttaa aacataattt taaaactgca
      181 aactacccaa gaaattatta ctttctacgt cacgtatttt gtactaatat ctttgtgttt
      241 acagtcaaat taattctaat tatctctcta acagccttgt atcgtatatg caaatatgaa
      301 ggaatcatgg gaaataggcc ctcttcctgc ccagatcttc aattccggcg cctagagaag
      361 gagtgagggc tggataaagg gaggatcgag gcggggtcga acgaggaggt tcaaggggga
      421 gagacggggc ggatggagga agaggaggcg gaggcttagg gtgtacaaag ggcttgaccc
      481 agggaggggg gtcaaaagcc aaggcttccc aggtcacgat gtaggggacc tggtctgggt
      541 gtccatgcgg gccaggtgaa aagaccttga tcttaacctg ggtgatgagg tctcggttaa
      601 aggtgccgtc tcgcggccat ccgacgttaa aggttggcca ttctgcagag cagaaggtaa
      661 cccaacgtct cttcttgaca tctaccgact ggttgtgagc gatccgctcg acatctttcc
      721 agtgac
//
