LOCUS       sequence-141930-        2889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L-lactate repressor (LldR) from E. coli MG1655.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-lldR
FEATURES             Location/Qualifiers
     source          1..2889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     39..57
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(61..104)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    107..126
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      969..1026
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1050..1067)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1221..1809)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1301..1320)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1992..2086)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2107..2766)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2681..2700
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
       61 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
      121 ttctagaatc tagagacgca taggtctcac atatgattgt tttacccaga cgcctgtcag
      181 acgaggttgc cgatcgtgtg cgggcgctga ttgatgaaaa aaacctggaa gcgggcatga
      241 agttgcccgc tgagcgccaa ctggcgatgc aactcggcgt atcacgtaat tcactgcgcg
      301 aggcgctggc aaaactggtg agtgaaggcg tgctgctcag tcgacgcggc ggcgggacgt
      361 ttattcgctg gcgtcatgac acatggtcgg agcaaaacat cgtccagccg ctaaaaacac
      421 tgatggccga tgatccggat tacagtttcg atattctgga agcccgctac gccattgaag
      481 ccagcaccgc atggcatgcg gcaatgcgcg ccacacctgg cgacaaagaa aagattcagc
      541 tttgctttga agcaacgcta agtgaagacc cggatatcgc ctcacaagcg gacgttcgtt
      601 ttcatctggc gattgccgaa gcctcacata acatcgtgct gctgcaaacc atgcgcggtt
      661 tcttcgatgt cctgcaatcc tcagtgaagc atagccgtca gcggatgtat ctggtgccac
      721 cggttttttc acaactgacc gaacaacatc aggctgtcat tgacgccatt tttgccggtg
      781 atgctgacgg ggcgcgtaaa gcaatgatgg cgcaccttag ttttgttcac accaccatga
      841 aacgattcga tgaagatcag gctcgccacg cacggattac ccgcctgccc ggtgagcata
      901 atgagcattc gagggagaaa aacgcataag gatcctcgaa gagacccatg ccgtctcatt
      961 agctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
     1021 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1081 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1141 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1201 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
     1261 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1321 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1381 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1441 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1501 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1561 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1621 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1681 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1741 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1801 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1861 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1921 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     1981 tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     2041 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     2101 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     2161 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     2221 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     2281 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg acacgaaaaa
     2341 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     2401 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     2461 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     2521 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     2581 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     2641 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     2701 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     2761 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     2821 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc
     2881 cacctgacg
//
