LOCUS       sequence-141931-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Hopx. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Hopx
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(491..509)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(492..509)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(527..543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(527..543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(540..562)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    551..567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(575..581,582..599,600..605))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    620..641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(758..775)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gccgcgggaa ttcgattatc tccggaggca gcacttgagg cgcttcctca gtatactgtc
       61 ccctcggagt gtcaggcggg aggcgtcttc ttcctcctct ccatccttag tcagacgcgc
      121 acggaccatg tcggcgcaga ccgcgagcgg ccccacggag gaccaggtgg agatcctgga
      181 gtacaacttc aacaaggtca acaagcaccc ggaccccacc acgctgtgcc tcatcgcagc
      241 cgaggcgggt ctcacggagg agcagacgca gaaatggttt aagcagcgcc tggcagagtg
      301 gcggcggtca gaaggcttgc cttcggaatg cagatctgtt acggactagg gagccaggcc
      361 cttgagcttg ctcttggaac tccatctctt cttccttccc tcggcttacc aatcactagt
      421 gaattcgcgg ccgcctgcag gtcgaccata tgggagagct cccaacgcgt tggatgcata
      481 gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca tagctgtttc
      541 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      601 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      661 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      721 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      781 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcgg
//
