LOCUS       sequence-141934-        4758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-T7RNAP
FEATURES             Location/Qualifiers
     source          1..4758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     39..57
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(61..104)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    107..126
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     misc_feature    2817..2837
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2838..2895
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2919..2936)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3090..3678)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3170..3189)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3861..3955)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3976..4635)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4550..4569
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
       61 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
      121 ttctagaatc tagagacgca taggtctcac atatgaacac gattaacatc gctaagaacg
      181 acttctctga catcgaactg gctgctatcc cgttcaacac tctggctgac cattacggtg
      241 agcgtttagc tcgcgaacag ttggcccttg agcatgagtc ttacgagatg ggtgaagcac
      301 gcttccgcaa gatgtttgag cgtcaactta aagctggtga ggttgcggat aacgctgccg
      361 ccaagcctct catcactacc ctactcccta agatgattgc acgcatcaac gactggtttg
      421 aggaagtgaa agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg caagaaatca
      481 agccggaagc cgtagcgtac atcaccatta agaccactct ggcttgccta accagtgctg
      541 acaatacaac cgttcaggct gtagcaagcg caatcggtcg ggccattgag gacgaggctc
      601 gcttcggtcg tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt gaggaacaac
      661 tcaacaagcg cgtagggcac gtctacaaga aagcatttat gcaagttgtc gaggctgaca
      721 tgctctctaa gggtctactc ggtggcgagg cgtggtcttc gtggcataag gaagactcta
      781 ttcatgtagg agtacgctgc atcgagatgc tcattgagtc aaccggaatg gttagcttac
      841 accgccaaaa tgctggcgta gtaggtcaag actctgagac tatcgaactc gcacctgaat
      901 acgctgaggc tatcgcaacc cgtgcaggtg cgctggctgg catctctccg atgttccaac
      961 cttgcgtagt tcctcctaag ccgtggactg gcattactgg tggtggctat tgggctaacg
     1021 gtcgtcgtcc tctggcgctg gtgcgtactc acagtaagaa agcactgatg cgctacgaag
     1081 acgtttacat gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa
     1141 tcaacaagaa agtcctagcg gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg
     1201 aggacatccc tgcgattgag cgtgaagaac tcccgatgaa accggaagac atcgacatga
     1261 atcctgaggc tctcaccgcg tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg
     1321 ctcgcaagtc tcgccgtatc agccttgagt tcatgcttga gcaagccaat aagtttgcta
     1381 accataaggc catctggttc ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt
     1441 caatgttcaa cccgcaaggt aacgatatga ccaaaggact gcttacgctg gcgaaaggta
     1501 aaccaatcgg taaggaaggt tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg
     1561 tcgataaggt tccgttccct gagcgcatca agttcattga ggaaaaccac gagaacatca
     1621 tggcttgcgc taagtctcca ctggagaaca cttggtgggc tgagcaagat tctccgttct
     1681 gcttccttgc gttctgcttt gagtacgctg gggtacagca ccacggcctg agctataact
     1741 gctcccttcc gctggcgttt gacgggtctt gctctggcat ccagcacttc tccgcgatgc
     1801 tccgagatga ggtaggtggt cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca
     1861 tctacgggat tgttgctaag aaagtcaacg agattctaca agcagacgca atcaatggga
     1921 ccgataacga agtagttacc gtgaccgatg agaacactgg tgaaatctct gagaaagtca
     1981 agctgggcac taaggcactg gctggtcaat ggctggctta cggtgttact cgcagtgtga
     2041 ctaagcgttc agtcatgacg ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag
     2101 tgctggaaga taccattcag ccagctattg attccggcaa gggtctgatg ttcactcagc
     2161 cgaatcaggc tgctggatac atggctaagc tgatttggga atctgtgagc gtgacggtgg
     2221 tagctgcggt tgaagcaatg aactggctta agtctgctgc taagctgctg gctgctgagg
     2281 tcaaagataa gaagactgga gagattcttc gcaagcgttg cgctgtgcat tgggtaactc
     2341 ctgatggttt ccctgtgtgg caggaataca agaagcctat tcagacgcgc ttgaacctga
     2401 tgttcctcgg tcagttccgc ttacagccta ccattaacac caacaaagat agcgagattg
     2461 atgcacacaa acaggagtct ggtatcgctc ctaactttgt acacagccaa gacggtagcc
     2521 accttcgtaa gactgtagtg tgggcacacg agaagtacgg aatcgaatct tttgcactga
     2581 ttcacgactc cttcggtacc attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg
     2641 aaactatggt tgacacatat gagtcttgtg atgtactggc tgatttctac gaccagttcg
     2701 ctgaccagtt gcacgagtct caattggaca aaatgccagc acttccggct aaaggtaact
     2761 tgaacctccg tgacatctta gagtcggact tcgcgttcgc gtaatcgaag agaccgtact
     2821 agtagcggcc gctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct
     2881 ttaaaaccga aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc
     2941 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     3001 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     3061 aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat
     3121 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3181 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     3241 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     3301 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     3361 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     3421 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     3481 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     3541 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3601 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3661 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3721 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3781 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3841 tctgacagct cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
     3901 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     3961 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     4021 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     4081 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     4141 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     4201 cacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     4261 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     4321 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     4381 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
     4441 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     4501 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     4561 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     4621 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     4681 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa
     4741 ctcgagtgcc acctgacg
//
