LOCUS       sequence-141938-        3020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 - SJM901 promoter (5' CTAT/3' GTAC fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-SJM901
FEATURES             Location/Qualifiers
     source          1..3020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..64
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     promoter        30..64
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             108..821
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(138..166)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     761..781
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      837..908
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      924..951
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      979..1036
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1060..1077)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1231..1819)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1311..1330)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2002..2096)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2117..2776)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2691..2710
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2938..2956
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2960..3003)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(3006..3020,1..5)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 tctagaacgt ctcaatctgg tctcactatt ttacagctag ctcagtccta ggtataatgc
       61 tagcgtacag agaccagatc ttttaacttt aagaaggaga tatacatatg cgtaaaggag
      121 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      181 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      241 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
      301 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
      361 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
      421 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
      481 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
      541 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
      601 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
      661 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
      721 ctgccctttc gaaagatccc aacgaaaaga gagatcacat ggtccttctt gagtttgtaa
      781 cagctgctgg gattacacat ggcatggatg aactatacaa ataatcgacc aggcatcaaa
      841 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      901 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatgt
      961 ttgccagaga cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     1021 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     1081 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1141 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1201 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     1261 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1321 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1381 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1441 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1501 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1561 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1621 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1681 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1741 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1801 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1861 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1921 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1981 gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt
     2041 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
     2101 cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
     2161 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
     2221 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     2281 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     2341 acacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     2401 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     2461 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     2521 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
     2581 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     2641 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     2701 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     2761 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     2821 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca
     2881 actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2941 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat
     3001 ttctggaatt cgcggccgct
//
