LOCUS       sequence-141941-        1244 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector with ubiquitin variant with reverted mutations 
            L69P and V70L (i53-DM mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Canny MD, Moatti N, Wan LCK, Fradet-Turcotte A, Krasner D, 
            Mateos-Gomez PA, Zimmermann M, Orthwein A, Juang YC, Zhang W, 
            Noordermeer SM, Seclen E, Wilson MD, Vorobyov A, Munro M, Ernst A, 
            Ng TF, Cho T, Cannon PM, Sidhu SS, Sicheri F, Durocher D
  TITLE     Inhibition of 53BP1 favors homology-dependent DNA repair and 
            increases CRISPR-Cas9 genome-editing efficiency.
  JOURNAL   Nat Biotechnol. 2018 Jan;36(1):95-102. doi: 10.1038/nbt.4021. Epub 
            2017 Nov 27.
  PUBMED    29176614
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.4021"; 
            journalName: "Nat Biotechnol"; date: "2018-01"; volume: "36"; issue:
            "1"; pages: "95-102"
COMMENT     Sequence Label: pMX-HA-UbvG08_MUT-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..1244
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..104
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    369..941
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    392..943
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(536..553)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     763..782
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(997..1018)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtgctagckc agctgccgac ccgggggtgg accatcctct agactgccgg atctagctag
       61 ttaattaagc caccatgtac ccatacgatg ttccagatta cgctgcagct gcaatgttga
      121 ttttcgtgaa aacccttacc gggaaaacca tcaccctcga ggttgaaccc tcggatacga
      181 tagaaaatgt aaaggccaag atccaggata aggaaggaat tcctcctgat cagcagagac
      241 tggcctttgc tggcaaatcg ctggaagatg gacgtacttt gtctgactac aatattctaa
      301 aggactctaa acttcatcta gtgttgagac ttcgttgagc ggccgctacg taaattccgc
      361 cccccccccc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
      421 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
      481 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
      541 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
      601 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
      661 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
      721 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
      781 ttcaacaagg agctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
      841 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
      901 aaccacgggg acgtggtttt cctttgaaaa cacgatgata atatggcmca accatggtga
      961 gcaaggcgag gagctgttca ccgggtggtg cccatcctgg tcgagctgga cggcgacgta
     1021 aacggccaca gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcagctgac
     1081 cctgagtcat ctgcacaccg gcaagctgcg tgcctgctta cctcgtgaca cctgaactac
     1141 gctgcatgct cagcgctacc cgaccatkga gcagccgact cctcagtcgc atgccgaagg
     1201 ytagttcaga gcacatcttc ttcaggacga cgtcacctac aaga
//
