LOCUS       sequence-141944-        1230 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral vector with ubiquitin variant that binds to and occludes 
            the ligand binding site of the 53BP1 Tudor domain (i53).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Canny MD, Moatti N, Wan LCK, Fradet-Turcotte A, Krasner D, 
            Mateos-Gomez PA, Zimmermann M, Orthwein A, Juang YC, Zhang W, 
            Noordermeer SM, Seclen E, Wilson MD, Vorobyov A, Munro M, Ernst A, 
            Ng TF, Cho T, Cannon PM, Sidhu SS, Sicheri F, Durocher D
  TITLE     Inhibition of 53BP1 favors homology-dependent DNA repair and 
            increases CRISPR-Cas9 genome-editing efficiency.
  JOURNAL   Nat Biotechnol. 2018 Jan;36(1):95-102. doi: 10.1038/nbt.4021. Epub 
            2017 Nov 27.
  PUBMED    29176614
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.4021"; 
            journalName: "Nat Biotechnol"; date: "2018-01"; volume: "36"; issue:
            "1"; pages: "95-102"
COMMENT     Sequence Label: pMX-HA-UbvG08-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..1230
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             77..103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(368..941,942..944,945..953)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(535..552)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     762..781
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gtggctgtka gctgccgacc cgggggtgga ccatcctcta gactgccgga tctagctagt
       61 taattaagcc accatgtacc catacgatgt tccagattac gctgcagctg caatgttgat
      121 tttcgtgaaa acccttaccg ggaaaaccat caccctcgag gttgaaccct cggatacgat
      181 agaaaatgta aaggccaaga tccaggataa ggaaggaatt cctcctgatc agcaragact
      241 ggcctttgct ggcaaatcgc tggaagatgg acgtactttg tctgactaca atattctaaa
      301 ggactctaaa cttcatcctc tgttgagact tcgttgagcg gccgctacgt aaattccgcc
      361 cccccccccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
      421 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
      481 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
      541 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
      601 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
      661 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
      721 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
      781 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
      841 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
      901 accacgggga cgtggttttc ctttgaaaac acgatgataa tatggccaca accatggtga
      961 gcaaggcgag gagctgttcm cggggtggtg ccatcctggt cgagctggac gcgacgtaaa
     1021 cgcyacaagt tcagcgtgtc gcgagggcga ggcgatgcac tacggcagct gaccctgagt
     1081 catctgcaca cggcagctgc cgtgctgaca cctcgtgaca ctgactacgc kcatgctcag
     1141 cgctaccgac cmtgagcaac gactctcagt cgcatgccag ctagtcagag cacwttctca
     1201 gacacgcctt acagacccgc cctaaggkka
//
