LOCUS       sequence-141954-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Jao LE, Wente SR, Chen W
  TITLE     Efficient multiplex biallelic zebrafish genome editing using a 
            CRISPR nuclease system.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 5.
  PUBMED    23918387
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Aug 5."
COMMENT     Sequence Label: pT3TS-nCas9n
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           39..81
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      97..106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             115..135
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 accctcacta aagggaacaa aagctggagc taagcttgct tgttcttttt gcagaagctc
       61 agaataaacg ctcaactttg gcagatctaa ctcgacgcca ccatggcttc tccacctaag
      121 aagaagagaa aggtgggaag catggataag aagtatagca tcggcctgga tattggaact
      181 aactccgtgg gttgggcagt gattacagac gactacaagg tccctagcaa gaaatttaag
      241 gtgctgggta acaccgacag gcacagcatc aagaaaaatc tgattggagc cctgctgttc
      301 ggttctggag agactgccga agcaacacgc ctgaaaagaa cagcaagaag gcgctatacc
      361 agaaggaaga atagaatctg ttacctgcag gagattttct ctaacgaaat ggctaaggtg
      421 gacgattcat tctttcatag gctggaggaa agtttcctgg tcgaggaaga taagaaacac
      481 gagcgccatc ctatctttgg aaacattgtg gacgaggtcg cctatcacga aaaataccca
      541 accatctatc atctgcgcaa gaaactggct gactctactg ataaagccga cctgagactg
      601 atctatctgg ctctggccca catgattaag ttcaggggtc attttctgat cgagggcgat
      661 ctgaaccccg acaattccga tgtggacaag ctgttcatcc agctggtcca gatttacaat
      721 cagctgtttg aggaaaaccc tattaatgct tccagagtgg acgcaaaagc tatcctgtca
      781 gccaggctgt ccaagtcacg cagactggag aacctgattg cacagctgcc cggagaaaag
      841 aggaacggtc tgtttggaaa tctgatcgct ctgagtctgg gcctgactcc taacttcaaa
      901 agcaattttg atctggctga ggacgcccaa
//
