LOCUS       sequence-142006-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human codon-optimized dCas9-DNMT3A fusion with 
            T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; 
            cloning backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-PuroR_v2
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             24..89
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             96..116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caggttggac cggtgccacc atggactata aggaccacga cggagactac aaggatcatg
       61 atattgatta caaagacgat gacgataaga tggccccaaa gaagaagcgg aaggtcggta
      121 tccacggagt cccagcagcc gacaagaagt acagcatcgg cctggccatc ggcaccaact
      181 ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc
      241 tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca
      301 gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac
      361 ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg
      421 acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc
      481 ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca
      541 tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct
      601 atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga
      661 accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc
      721 tgttcgagga
//
