LOCUS       sequence-142007-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human codon-optimized dCas9-DNMT3A fusion with 
            T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; 
            cloning backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-PuroR_v2
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttagcagact tcctctgccc tctccactgc cgaattcggg ccggccggac acacacgcaa
       61 aatactcctt cagcggagcg aagaggtggc ggatgactgg cacgctccat gaccggccca
      121 gcagtctctg cctcgccaag cggctcatgt tggagacgtc agtatagtgg actgggaaac
      181 caaataccct ttccatttca gtgcaccata agatgtcctc tttctcattc atgaacacag
      241 gaaaatgctg gtctttgccc tgctttatgg agtttgacct cgtagtaatg gtcctcactt
      301 tgctgaactt ggctatcctg ccatgctcca gacactcctg cagctccagc ttatcattca
      361 cagtggatgc caacggcctg ttcataccgg gaaggttacc ccagaagtag cgggccctgt
      421 gtgcagctga cacttctttg gcatcaatca tcacagggtt ggactcgaga aatcgcgaga
      481 tgtccctctt gtcactaacg cccatggcca ccacattctc aaagagccag aagaaggggc
      541 gatcatctcc ctccttgggc cgcgcatcat gcaggaggcg gtagaactca aagaagagcc
      601 ggccagtgcc ctcgtagagg cccttgcgag cagggttgac gatggagagg tcattgcagg
      661 gactgccccc aatcaccaga tcgaatgggc cccactcctg gatatgcttc tgtgtgacgc
      721 tgcggacgtc c
//
