LOCUS       sequence-142008-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pcin/tet-w reporter plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Chen Y, Kim JK, Hirning AJ, Josic K, Bennett MR
  TITLE     SYNTHETIC BIOLOGY. Emergent genetic oscillations in a synthetic 
            microbial consortium.
  JOURNAL   Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.
  PUBMED    26315440
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-08-28- 28"; volume: "349"; 
            issue: "6251"; pages: "986-9"
COMMENT     Sequence Label: pCH117
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    154..172
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             373..402
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    556..574
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tcgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc
       61 ggtatggatg cggcgggacc agagctcagt gcgctcgagc ttcccaacct taccagaggg
      121 cgccccagct ggcaattccg acgtctgtgt ggatccctat cagtgataga gattcacaca
      181 gggccctcgg acaccgagga gaatgtcaag aggcgaacac acaacgtctt ggagcgccag
      241 aggaggaacg agctaaaacg gagctttttt gccctgcgtg accagatccc ggagttggaa
      301 aacaatgaaa aggcccccaa ggtagttatc cttaaaaaag ccacagcata catcctgtcc
      361 gtccaagcag aggagcaaaa gctcatttct gaagaggact tgttgcggaa acgacgagaa
      421 cagttgaaac acaaacttga acagctacgg aactcttgtg cgtaaggaaa agtaaggaaa
      481 acgattcctt ctaacagaaa tgtcctgagc aatcacctat gaactgtcga gggggcctat
      541 ctgagggaat ttacttccct atcagtgata gagatactga gcacatccct atcagtgata
      601 gagatgcatg ccacttaaaa aggagatcaa caatgaaagc aattttcgta ctgaaacatc
      661 ttaatcatgc taaggagaaa tactagtatg cgtaaaggcg aagagctgtt cactggtgtc
      721 gtccctattc tggtggaact g
//
