LOCUS       sequence-142011-         516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of eNpHR 3.0 fused to EYFP driven by humanized 
            Synapsin promoter for optogenetic inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 516)
  TITLE     eNpHR3.0-EYFP
REFERENCE   2  (bases 1 to 516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAAV-hSyn-eNpHR 3.0-EYFP
FEATURES             Location/Qualifiers
     source          1..516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(76..327)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(118..139)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     467..486
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattctc attacacctc
       61 gttctcgtag cagaacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
      121 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      181 gtagctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      241 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
      301 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
      361 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
      421 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
      481 gtcgtccttg aagaagatgg tgcgctcctg gacgta
//
