LOCUS       sequence-142017-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains sgRNA which targets GFPmut3b. Under an aTc inducible 
            promoter. Suicide vector inserts into slr0230 site of Synechocystis.
            Carries kanamycin resistance. Propogates in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Yao L, Cengic I, Anfelt J, Hudson EP
  TITLE     Multiple Gene Repression in Cyanobacteria Using CRISPRi.
  JOURNAL   ACS Synth Biol. 2015 Dec 28.
  PUBMED    26689101
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 28."
COMMENT     Sequence Label: pMD19T-slr0230-PL22-sgRNANT1-KmR
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    93..111
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    118..136
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    137..158
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ctttttcaag ttgataacgg actagcctta ttttaacttg ctatttctag ctctaaaaca
       61 attcttgttg aattagatgg tagctcccag tatctctatc actgataggg atgtcaatct
      121 ctatcactga tagggactct agaagcggcc gcgaattcca tcagccacag ggtaaatggt
      181 tgtgcccaac ttttgagcca catcggccac ccggaggtca tccaagaagc gagtatcatc
      241 atgtttcaac atcagagcag gcaatagaat accatcccct aaatctctgc ctgccaattt
      301 agcgattaaa tcctgccccg tcaatagtcc cgtcaccgta atttcctgac cccaatagtc
      361 actgtttaag ggcgctaaat taactgttaa accctttacc tgatttaact gttccaccag
      421 tagctcaaac gcttgttcta cagcattgcc cactacccag gtcaacgtct tggcttcggc
      481 gatcgccggg gggagaaatt ccgcagcttg ttgttgaaac tccttaatga attgacggat
      541 agaccctacc ccgttgccaa tttggggata gtcttcgtaa tggctttccg gtggcaaagg
      601 ttgacgggca atgagaaacc attcatcggc caaccaaaca aagttaccac ctagttgctg
      661 ggcaaattcc ttttgcaaag cttgaacctg ggcaattacc tccgtggctt tagcttgacc
      721 aacggggctt aactcatctt cctggggacg aaaacgggtt agacccaccg gcaccaccgc
      781 cactgaaatc accgctgggg tttccccttg gtgaaactgg gctaagtccc tcaaagtttg
      841 ctctaagtg
//
