LOCUS       sequence-142019-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains sgRNA which targets GFPmut3b. Under an aTc inducible 
            promoter. Suicide vector inserts into slr0230 site of Synechocystis.
            Carries kanamycin resistance. Propogates in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Yao L, Cengic I, Anfelt J, Hudson EP
  TITLE     Multiple Gene Repression in Cyanobacteria Using CRISPRi.
  JOURNAL   ACS Synth Biol. 2015 Dec 28.
  PUBMED    26689101
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 28."
COMMENT     Sequence Label: pMD19T-slr0230-PL22-sgRNANT1-KmR
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(9..31)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     131..150
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     344..366
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     358..375
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     359..375
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
       61 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
      121 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
      181 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
      241 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
      301 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      361 aaaacgacgg ccagtgaatt agaactcggt acgcgcggat cttccagaga ttgccacctg
      421 acgtctaaga aaccataacc gttgtccctt ttgtttcatc gaccaacaac cgccggggaa
      481 acgggagagt ttgtattaca aagatgatga ctatcgcctg agttttctct acggcagtta
      541 tctcactttg actaatttga gtgccaagga atggcaacgc atagaacagt taaggctttc
      601 tcccctctac gtttccatcc atgccacgga agcatcggtg cgggaaagat tgttaaaaaa
      661 tcatcgggcc ggtcagattc ttgaccagtt ggcttggttc caagctagaa gattacagat
      721 ccacgcccag gtggtggttt gtcctggtgt taatgatggt aagcacttag agcaaacttt
      781 gagggactta gcccagtttc accaagggga aaccccagcg gtgatttcag tggcggtggt
      841 gccggtgggt ctaacccgtt ttcgtcccca ggaagatgag ttaagccccg ttggtcaagc
      901 taaagccacg gaggtaattg cccaggttca agctttgcaa aaggaatttg cccagcaact
      961 aggtggtaac tttgtttggt tggccgatga atggtttctc attgcccgtc a
//
