LOCUS       sequence-142025-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Tgif1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Tgif1
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(760..778)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(761..778)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(796..812)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(796..812)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(809..831)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    820..836
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(844..850,851..868,869..874))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    889..910
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcggccgcgg gaattcgatt tgggaagaga gaggtgcgag ggcgtctaaa gtattgcaca
       61 gtcttggaac ataaagaaga ggagtctgga ggagaagatc acatgatact gggaaccgtc
      121 tcacattatg agaaactacc aagaaacatt cgaaaagaaa accctttgtt tctacagtag
      181 ctttagtttg tagttcttgg aatgactcta ttccacttaa gacatcttag taacaggaag
      241 cttcgtctga gcaatcccat gtgcaaatat taatagaaaa atatataaaa taatgcatct
      301 cttgccatca cccccggcaa tcaggaccgt attttggaga actgttttgt ttgaaaaaac
      361 ggttaagctg tgagtttggc ctgaagctcc atctctgccg ctcgtttgag tgcaacatcc
      421 actagaagct ggaatccact aaaatcctgg ttgaggtctg gtggagtagg gggaggagtg
      481 ttgaaaagac cactctgagg gtttggactg ggtccagatt tgcaagtgtc ctctgggtac
      541 acgagagagg tgtctgtaaa gttgctgggt gctatttgcg gtacatctgt actctgtccc
      601 acaccaatcg gctgacagag agagaaaggc ccatccttca atgcagtcac agtggtatgg
      661 cagatcactg acgggcgaga atcactagtg aattcgcggc cgcctgcagg tcgaccatat
      721 gggagagctc ccaacgcgtt ggatgcatag cttgagtatt ctatagtgtc acctaaatag
      781 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      841 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      901 actcacatta attgcgttgc gctcactgcc
//
