LOCUS       sequence-142027-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Tgif1. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Tgif1
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      726..735
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      730..739
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(769..788)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(770..788)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(795..812)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(795..811)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(804..826)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ggagctctcc atatggtcga cctgcaggcg gccgcgaatt cactagtgat tctcgcccgt
       61 cagtgatctg ccataccact gtgactgcat tgaaggatgg gcctttctct ctctgtcagc
      121 cgattggtgt gggacagagt acagatgtac cgcaaatagc acccagcaac tttacagaca
      181 cctctctcgt gtacccagag gacacttgca aatctggacc cagtccaaac cctcagagtg
      241 gtcttttcaa cactcctccc cctactccac cagacctcaa ccaggatttt agtggattcc
      301 agcttctagt ggatgttgca ctcaaacgag cggcagagat ggagcttcag gccaaactca
      361 cagcttaacc gttttttcaa acaaaacagt tctccaaaat acggtcctga ttgccggggg
      421 tgatggcaag agatgcatta ttttatatat ttttctatta atatttgcac atgggattgc
      481 tcagacgaag cttcctgtta ctaagatgtc ttaagtggaa tagagtcatt ccaagaacta
      541 caaactaaag ctactgtaga aacaaagggt tttcttttcg aatgtttctt ggtagtttct
      601 cataatgtga gacggttccc agtatcatgt gatcttctcc tccagactcc tcttctttat
      661 gttccaagac tgtgcaatac tttagacgcc ctcgcacctc tctcttccca aatcgaattc
      721 ccgcggccgc catggcggcc gggagcatgc gacgtcgggc ccaattcgcc ctatagtgag
      781 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      841 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      901 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccc
//
