LOCUS       sequence-142028-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mKate2 (codon adapted for Streptococcus pneumoniae); under
            control of CSP inducible promoter PssbB. Plasmid integrates in S. 
            pneumoniae bgaA locus by double cross-over..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Beilharz K, van Raaphorst R, Kjos M, Veening JW
  TITLE     Red fluorescent proteins for gene expression and protein 
            localization studies in Streptococcus pneumoniae and efficient 
            transformation with Gibson assembled DNA.
  JOURNAL   Appl Environ Microbiol. 2015 Aug 7. pii: AEM.02033-15.
  PUBMED    26253684
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2015 Aug 7. pii: AEM.02033-15."
COMMENT     Sequence Label: pKB01-PssbB-mKate2
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccgtcctgtg gatctatcga tgcatgcagc cggtaccatc acaagcactt tgggacgttc
       61 tcccttagtg cttttttgat ttctcatagg ccggccgcct agaattccaa tcccgggtaa
      121 agcttccagc ggccgcctca ggatattgca gacacagtag tctatgtgac cagtcagcct
      181 cgccgtgttc agattacaga tatgaccatt atggccaatc aacaggcgac aggtttcatg
      241 attcataaaa aataagaaat ttcctcgaaa agttacaaat ttctgtaact ttttttgatt
      301 tcctacgaat agataagtag gaggaagaaa atatgtataa taaagttatc atgattgggc
      361 gtttaacgtc tacaccggat ccattgatat catattctag aggaggaaaa ttaatgtcag
      421 aacttatcaa ggaaaatatg cacatgaaat tgtacatgga aggaacagta aataatcacc
      481 actttaaatg tacctcagaa ggagaaggaa aaccatatga aggtactcaa accatgcgta
      541 ttaaggccgt tgaaggtgga ccattgcctt ttgcctttga tattcttgct acatctttta
      601 tgtacggatc aaagactttt atcaatcata cccaaggtat cccagatttc tttaaacagt
      661 catttcctga aggatttaca tgggaacgtg tcacaactta tgaagatggt ggagtattga
      721 cagcaactca agatacatct ttgcaagatg gttgtcttat ctacaatgta aagatccgtg
      781 gagttaattt tccaagtaat ggtcctgtta tgcagaaaaa gacccttgga tgggaagcat
      841 ctaccgaaac attatatcct gccgatggtg gattggaagg tc
//
