LOCUS       sequence-142040-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Use to synthesize riboprobes for in situ hybridization (ISH) probe 
            for Tshz3. Not for gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Parrilla M, Chang I, Degl'Innocenti A, Omura M
  TITLE     Expression of homeobox genes in the mouse olfactory epithelium.
  JOURNAL   J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. 
            Epub 2016 Jun 19.
  PUBMED    27243442
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.24051";
            journalName: "J Comp Neurol"; date: "2016-10-1- 1"; volume: "524"; 
            issue: "14"; pages: "2713-39"
COMMENT     Sequence Label: pISH-Tshz3
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(764..782)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(765..782)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(800..816)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(800..816)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(813..835)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    824..840
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cggccgcggg aattcgattg gtccgtgata atggctgtgc tgccgctgag acctccgctc
       61 aggggcttca ccagctcctt gccattctct acgggctctg cggaggggct cgcctccttg
      121 cacgcatctc ttggtgggct ggggctattc tcctgacttt tgaagctgcc gtcgctggag
      181 gcctccatct tgatgggttc gctctgctcg ctgctgcacg gggacggcgt ggcccgcttg
      241 ggtggcgaga gcttgccttc tggctctttc attttctcct caactttggc aactttctcc
      301 gtgacttttt tcaccagttc ctccatggcg tgaaagtttg tcttgggcat gggagaggtc
      361 tggctgctgg gtggagagac cagggtctgg gtttttgtgg gagacacgat ttcgctgttg
      421 ccaaacatgg gtttcagggg cgtgctcttc cccgaggaac ccagggacaa cttcatcatg
      481 ttggggagct ggtaggcagc gtggatgctg ggatagccgc cccagctggg agtgccattc
      541 tgagccttgt tgatggcaga cgttactgtg ttctcaaggg atttgaggat atctagtcca
      601 cctttagggc tctcttctag gtcgttttca gtcaaatagt ggtacttgga agaaatatca
      661 tacttctcct cctcttcact gggaatcact agtgaattcg cggccgcctg caggtcgacc
      721 atatgggaga gctcccaacg cgttggatgc atagcttgag tattctatag tgtcacctaa
      781 atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      841 ttccacacaa cata
//
